<?xml version='1.0' encoding='utf-8'?>
<emdEntry accessCode="9899" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2020-05-20</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2019-05-05</depositionDate>
        <depositionSite>PDBj</depositionSite>
        <processingSite>PDBj</processingSite>
        <headerReleaseDate>2019-06-19</headerReleaseDate>
        <mapReleaseDate>2019-06-19</mapReleaseDate>
        <obsoletedDate>2020-03-04</obsoletedDate>
        <supersededByList>
            <entry>EMD-30036</entry>
        </supersededByList>
        <title>cryo-EM structure of an archaeal Ribonuclease P</title>
        <authors>Wan F, Lan P, Wu J, Lei M</authors>
        <fittedPDBEntryIdList>
            <fittedPDBEntryId>6k0a</fittedPDBEntryId>
        </fittedPDBEntryIdList>
        <primaryReference published="true">
            <journalArticle>
                <authors>Wan F, Wang Q, Tan J, Tan M, Chen J, Shi S, Lan P, Wu J, Lei M</authors>
                <articleTitle>Cryo-electron microscopy structure of an archaeal ribonuclease P holoenzyme.</articleTitle>
                <journal>Nat Commun</journal>
                <volume>10</volume>
                <firstPage>2617</firstPage>
                <lastPage>2617</lastPage>
                <year>2019</year>
                <externalReference type="pubmed">31197137</externalReference>
                <externalReference type="doi">doi:10.1038/s41467-019-10496-3</externalReference>
                <externalReference type="issn">2041-1723</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="108001" type="map">emd_9899.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>300</numRows>
            <numColumns>300</numColumns>
            <numSections>300</numSections>
        </dimensions>
        <origin>
            <originRow>-26</originRow>
            <originCol>-26</originCol>
            <originSec>-26</originSec>
        </origin>
        <limit>
            <limitRow>273</limitRow>
            <limitCol>273</limitCol>
            <limitSec>273</limitSec>
        </limit>
        <spacing>
            <spacingRow>300</spacingRow>
            <spacingCol>300</spacingCol>
            <spacingSec>300</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">396.00003</cellA>
            <cellB units="A">396.00003</cellB>
            <cellC units="A">396.00003</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.085028276</minimum>
            <maximum>0.17260739</maximum>
            <average>0.0006357505</average>
            <std>0.005365306</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">1.32</pixelX>
            <pixelY units="A">1.32</pixelY>
            <pixelZ units="A">1.32</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.02</contourLevel>
    </map>
    <sample>
        <numComponents>8</numComponents>
        <name>cryo-EM structure of an archaeal Ribonuclease P</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>cryo-EM structure of an archaeal Ribonuclease P</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="2190">Methanocaldococcus jannaschii</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="562">Escherichia coli</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>Ribonuclease P protein component 2</sciName>
                <molWtTheo units="MDa">0.015972156</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="243232">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</sciSpeciesName>
                    <sciSpeciesStrain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</sciSpeciesStrain>
                    <numCopies>2</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>Ribonuclease P protein component 3</sciName>
                <molWtTheo units="MDa">0.027394783</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="243232">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</sciSpeciesName>
                    <sciSpeciesStrain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</sciSpeciesStrain>
                    <numCopies>2</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>protein</entry>
                <sciName>Ribonuclease P protein component 1</sciName>
                <molWtTheo units="MDa">0.010910179</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="243232">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</sciSpeciesName>
                    <sciSpeciesStrain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</sciSpeciesStrain>
                    <numCopies>2</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="5">
                <entry>protein</entry>
                <sciName>Ribonuclease P protein component 4</sciName>
                <molWtTheo units="MDa">0.015610048</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="243232">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</sciSpeciesName>
                    <sciSpeciesStrain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</sciSpeciesStrain>
                    <numCopies>2</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="6">
                <entry>protein</entry>
                <sciName>50S ribosomal protein L7Ae</sciName>
                <molWtTheo units="MDa">0.012703843</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="243232">Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440)</sciSpeciesName>
                    <sciSpeciesStrain>ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440</sciSpeciesStrain>
                    <numCopies>2</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="7">
                <entry>nucleic-acid</entry>
                <sciName>RPR</sciName>
                <molWtTheo units="MDa">0.083581586</molWtTheo>
                <nucleic-acid>
                    <sciSpeciesName ncbiTaxId="2190">Methanocaldococcus jannaschii</sciSpeciesName>
                    <syntheticFlag>false</syntheticFlag>
                    <sequence>GGAGGGGGCUGGUGACUUUCCCCUCUUUAAGAGGGGAGGAAGUUCCGCCCACCCCAUUUAUGGGCAGCGUCCCCUGAGAA GGGGCGGGAGAUGCAGCAGAAACGACACGGCUCCGGAAGAGAUGACGAUGAUAGUGAAAGUUGAGGACUUCCGGAGAACC GGUGAAACGGGCAUCUCCCCUGCCCGGGGUGCAAGCCGGUUUCGGCGCUUAGCCGAAUGUCACCGAAAUUACAGAAGGCG GGCUAUAGCCCCCAUUUU</sequence>
                    <class>RNA</class>
                    <structure>OTHER</structure>
                </nucleic-acid>
            </sampleComponent>
            <sampleComponent componentID="8">
                <entry>ligand</entry>
                <sciName>ZINC ION</sciName>
                <molWtTheo units="MDa">6.5409e-05</molWtTheo>
                <ligand>
                    <numCopies>2</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </ligand>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <details />
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">50.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON III (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>8</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <resolutionByAuthor>4.6</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C2</appliedSymmetry>
            <numProjections>84800</numProjections>
        </singleParticle>
    </processing>
</emdEntry>