<?xml version='1.0' encoding='utf-8'?>
<emdEntry accessCode="9364" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2021-02-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-12-19</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2021-02-10</headerReleaseDate>
        <obsoletedDate>2021-02-10</obsoletedDate>
        <title>The structure of TRIM5alpha trimer in complex with HIV-1 capsid tubes</title>
        <authors>Skorupka KA, Ganser-Pornillos BK, Pornillos O</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Skorupka KA, Pornillos O</authors>
                <articleTitle>The structure of the TRIM5aplha dimer assembled on the CA</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="8389" type="map">emd_9364.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>128</numRows>
            <numColumns>128</numColumns>
            <numSections>128</numSections>
        </dimensions>
        <origin>
            <originRow>0</originRow>
            <originCol>0</originCol>
            <originSec>0</originSec>
        </origin>
        <limit>
            <limitRow>127</limitRow>
            <limitCol>127</limitCol>
            <limitSec>127</limitSec>
        </limit>
        <spacing>
            <spacingRow>128</spacingRow>
            <spacingCol>128</spacingCol>
            <spacingSec>128</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">373.76</cellA>
            <cellB units="A">373.76</cellB>
            <cellC units="A">373.76</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-12.998528</minimum>
            <maximum>19.72153</maximum>
            <average>-0.15605806</average>
            <std>0.7392354</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">2.92</pixelX>
            <pixelY units="A">2.92</pixelY>
            <pixelZ units="A">2.92</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">1.0</contourLevel>
        <annotationDetails>The structure of TRIM5alpha trimer in complex with HIV-1 capsid tubes, primary map</annotationDetails>
    </map>
    <sample>
        <numComponents>2</numComponents>
        <name>Ternary complex of TRIM5alpha with HIV-1 CA protein</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Ternary complex of TRIM5alpha with HIV-1 CA protein</sciName>
                <molWtTheo units="MDa">0.07</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="60710">Chlorocebus pygerythrus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="7108">Spodoptera frugiperda</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>Tripartite motif-containing protein 5</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="60710">Chlorocebus pygerythrus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="7108">Spodoptera frugiperda</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <details>manual freezning. </details>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">1.3</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>OTHER</illuminationMode>
            <detector>FEI FALCON II (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
        <fitting>
            <pdbEntryIdList>
                <pdbEntryId>4TN3</pdbEntryId>
            </pdbEntryIdList>
            <refProtocol>rigid body</refProtocol>
        </fitting>
        <specimenPreparation>
            <specimenState>threeDArray</specimenState>
            <buffer>
                <ph>10</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>subtomogramAveraging</method>
        <reconstruction>
            <algorithm>BACK PROJECTION</algorithm>
            <ctfCorrection>CTF correction was performed on 2D images by IMOD</ctfCorrection>
            <resolutionByAuthor>26.7</resolutionByAuthor>
            <resolutionMethod>FSC 0.5 CUT-OFF</resolutionMethod>
        </reconstruction>
        <subtomogramAveraging>
            <appliedSymmetry>C2</appliedSymmetry>
            <numSubtomograms>3204</numSubtomograms>
        </subtomogramAveraging>
    </processing>
</emdEntry>