2021-02-01 OBS 2018-07-11 RCSB RCSB 2021-02-10 2021-02-10 Suppressed Suppressed X, Suppressed X, Suppressed X, Suppressed X 6e2k Suppressed X, Suppressed X, Suppressed X, Suppressed X, Suppressed X, Suppressed X, Suppressed X Suppressed Suppressed emd_8963.map.gz Image stored as Reals 236 236 236 0 0 0 235 235 235 236 236 236 250.15999 250.15999 250.15999 90.0 90.0 90.0 X Y Z -0.045405697 0.100587204 -4.301027e-05 0.0047599208 1
1.06 1.06 1.06 0.0234 Suppressed 2 Suppressed
Suppressed
cellular-component Suppressed 1
Suppressed
Suppressed Suppressed false Suppressed protein Suppressed 1e-06 Suppressed 12 false Suppressed ETHANE FEI VITROBOT MARK IV
After application of sample to the C-flat holey carbon grid, sample was blotted with Whatman no. 1 filter paper for ~4s, blot force of 3, and 100 % humidity at 22oC..
FIELD EMISSION GUN 45.0 GIF Quantum LS BRIGHT FIELD SPOT SCAN GATAN K2 SUMMIT (4k x 4k) 2.7 FEI TITAN KRIOS OTHER 300 130000 Model-map cross correlation REAL
Initial local fitting was done using UCSF Chimera. Multiple rounds of automated (Phenix) and manual (Coot) model building were performed. The model was validated in Molprobity(part of Phenix package). Model-map cross correlation score as well as EMRinger score were obtained in Phenix. RMSD values for C-alphas were calculated in UCSF Chimera.
 particle 0.6 6.4
NaCl was diluted from 1 M to 200 mM immediately before freezing. HEPES pH 7.5 buffer was diluted with MES pH 6.0 buffer thus adjusting pH to 6.4.
 singleParticle RELION 4.2 FSC 0.143 CUT-OFF D6 25236