2021-02-01 OBS RCSB RCSB RCSB 2018-07-11 2021-02-10 2021-02-10 2021-02-01 Suppressed Suppressed Suppressed Suppressed Suppressed Suppressed Suppressed Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed Suppressed EMD-8963 associated EM volume
Suppressed
 EMD-8964 other EM volume
Suppressed
 6e2k FULLOVERLAP Suppressed Suppressed 0 1
Suppressed
Suppressed Suppressed 1 Suppressed Suppressed Suppressed 1e-06 12 Suppressed LEVO X singleParticle particle 0.6 6.4 12.5 C8H18N2O4S HEPES 37.5 C6H13NO4S MES 200.0 NaCl sodium chloride
NaCl was diluted from 1 M to 200 mM immediately before freezing. HEPES pH 7.5 buffer was diluted with MES pH 6.0 buffer thus adjusting pH to 6.4.
 C-flat-2/2 GLOW DISCHARGE ETHANE FEI VITROBOT MARK IV
After application of sample to the C-flat holey carbon grid, sample was blotted with Whatman no. 1 filter paper for ~4s, blot force of 3, and 100 % humidity at 22oC..
FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.7 130000.0 GIF Quantum LS 20 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 1 10.0 45.0 1 49952 CTFFIND 4 RELION
2D class averages of the auto-picked particles were calculated and used to generate a de novo D6 symmetric initial model in EMAN2.
 D6 4.2 FSC 0.143 CUT-OFF RELION 25236 MAXIMUM LIKELIHOOD RELION MAXIMUM LIKELIHOOD RELION emd_8963.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 236 236 236 0 0 0 236 236 236 250.15999 250.15999 250.15999 90.0 90.0 90.0 X Y Z -0.045405697 0.100587204 -4.301027e-05 0.0047599208 1.06 1.06 1.06 0.0234 AUTHOR Suppressed OTHER
Initial local fitting was done using UCSF Chimera. Multiple rounds of automated (Phenix) and manual (Coot) model building were performed. The model was validated in Molprobity(part of Phenix package). Model-map cross correlation score as well as EMRinger score were obtained in Phenix. RMSD values for C-alphas were calculated in UCSF Chimera.
Model-map cross correlation REAL emd_8963_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 236 236 236 0 0 0 236 236 236 250.15999 250.15999 250.15999 90.0 90.0 90.0 X Y Z -0.005797484 0.024512978 -3.036325e-05 0.0015215686 1.06 1.06 1.06 AUTHOR Suppressed