2021-02-01 OBS 2018-06-13 RCSB RCSB 2021-02-10 2021-02-10 Negative stain EM map of the MTA-HDAC-RBBP (MHR) complex Silva APG, Low JKK, Tabar MS, Torrado M, Webb SR, Schmidberger JW, Brillault L, Shepherd NE, Landsberg MJ, Mackay JP Silva APG, Low JKK, Tabar MS, Torrado M, Webb SR, Parker BL, Sana M, Smits C, Schmidberger JW, Brillault L, Williams Jr DC, Hake SB, Shepherd NE, Landsberg MJ, Mackay JP The Nucleosome Remodeling and Deacetylase complex has an asymmetric, dynamic and modular architecture To Be Published 0353 emd_8906.map.gz Image stored as Reals 128 128 128 0 0 0 127 127 127 128 128 128 357.12 357.12 357.12 90.0 90.0 90.0 X Y Z -0.12580065 0.28989676 0.0022528672 0.02393168 1
2.79 2.79 2.79 0.0487 Negative stain EM map of the MHR complex. 4 MHR complex
Recombinantly purified complex composed of FLAG-MTA2, HA-HDAC1 and HA-RBBP7.
protein MHR complex 0.45
Recombinantly purified complex composed of FLAG-MTA2, HA-HDAC1 and HA-RBBP7.
Homo sapiens false Homo sapiens HEK293 protein Metastasis-associated protein MTA2 Mus musculus false Homo sapiens protein Histone deacetylase 1 Homo sapiens false Homo sapiens protein Histone-binding protein RBBP7 Homo sapiens false Homo sapiens NONE LAB6 10.0 BRIGHT FIELD FLOOD BEAM OTHER FEI TECNAI 12 OTHER 120 469 particle 0.007 7.5 Sample (5 microliters) was applied to a glow-discharged, carbon-coated 400-mesh copper grid (GSCu400CC, ProSciTech). After 2 min incubation time, the grid was blotted and washed with ten drops of distilled water, blotting on a filter paper in between washes. The grid was then washed in a drop of 1 % (w/v) uranyl acetate, blotted and subsequently incubated in another drop of 1% (w:v) uranyl acetate solution for 30 s, blotted and allowed to air dry at room temperature. singleParticle RELION 32.0 FSC 0.143 CUT-OFF C1 7043