<?xml version='1.0' encoding='utf-8'?>
<emdEntry accessCode="8905" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2021-02-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-06-13</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2021-02-10</headerReleaseDate>
        <obsoletedDate>2021-02-10</obsoletedDate>
        <title>Negative stain EM map of the NuDe, a NuRD subcomplex</title>
        <authors>Silva APG, Low JKK, Tabar MS, Torrado M, Webb SR, Schmidberger JW, Brillault L, Shepherd NE, Landsberg MJ, Mackay JP</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Silva APG, Low JKK, Tabar MS, Torrado M, Webb SR, Parker BL, Sana M, Smits C, Schmidberger JW, Brillault L, Williams Jr DC, Hake SB, Shepherd NE, Landsberg MJ, Mackay JP</authors>
                <articleTitle>The Nucleosome Remodeling and Deacetylase complex has an asymmetric, dynamic and modular architecture</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="19653" type="map">emd_8905.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>170</numRows>
            <numColumns>170</numColumns>
            <numSections>170</numSections>
        </dimensions>
        <origin>
            <originRow>0</originRow>
            <originCol>0</originCol>
            <originSec>0</originSec>
        </origin>
        <limit>
            <limitRow>169</limitRow>
            <limitCol>169</limitCol>
            <limitSec>169</limitSec>
        </limit>
        <spacing>
            <spacingRow>170</spacingRow>
            <spacingCol>170</spacingCol>
            <spacingSec>170</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">408.00003</cellA>
            <cellB units="A">408.00003</cellB>
            <cellC units="A">408.00003</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.078712486</minimum>
            <maximum>0.14078417</maximum>
            <average>0.00013098281</average>
            <std>0.008607291</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">2.4</pixelX>
            <pixelY units="A">2.4</pixelY>
            <pixelZ units="A">2.4</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.0168</contourLevel>
        <annotationDetails>Negative stain EM map of the Nucleosome Remodeling and Deacetylase Complex</annotationDetails>
    </map>
    <sample>
        <numComponents>6</numComponents>
        <name>Nucleosome Deacetylase (NuDe) complex</name>
        <details>Purified endogenously from MEL cells.</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Nucleosome Deacetylase (NuDe) complex</sciName>
                <molWtExp units="MDa">0.7</molWtExp>
                <details>Purified endogenously from MEL cells.</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource />
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>Metastasis-associated protein MTA2</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>Histone deacetylase 1</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>protein</entry>
                <sciName>Histone-binding protein RBBP4</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="5">
                <entry>protein</entry>
                <sciName>Methyl-CpG-binding protein MBD3</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="6">
                <entry>protein</entry>
                <sciName>GATA zinc finger domain-containing protein 2A</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>NONE</cryogenName>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">10.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>OTHER</detector>
            <microscope>FEI TECNAI F30</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition>
            <numDigitalImages>400</numDigitalImages>
        </imageAcquisition>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>7.5</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>31.0</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C1</appliedSymmetry>
            <numProjections>13092</numProjections>
        </singleParticle>
    </processing>
</emdEntry>