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    <admin>
        <lastUpdate>2021-02-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-06-13</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2021-02-10</headerReleaseDate>
        <obsoletedDate>2021-02-10</obsoletedDate>
        <title>Negative stain EM map of the Nucleosome Remodeling and Deacetylase (NuRD) complex</title>
        <authors>Silva APG, Low JKK, Tabar MS, Torrado M, Webb SR, Schmidberger JW, Brillault L, Shepherd NE, Landsberg MJ, Mackay JP</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Silva APG, Low JKK, Tabar MS, Torrado M, Webb SR, Parker BL, Sana M, Smits C, Schmidberger JW, Brillault L, Williams Jr DC, Hake SB, Shepherd NE, Landsberg MJ, Mackay JP</authors>
                <articleTitle>The Nucleosome Remodeling and Deacetylase complex has an asymmetric, dynamic and modular architecture</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
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            <numSections>180</numSections>
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        <origin>
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            <limitRow>179</limitRow>
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            <cellB units="A">390.6</cellB>
            <cellC units="A">390.6</cellC>
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            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
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        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
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            <axisOrderSlow>Z</axisOrderSlow>
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            <maximum>0.17932773</maximum>
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        <details />
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        <annotationDetails>Negative stain EM map of the Nucleosome Remodeling and Deacetylase Complex</annotationDetails>
    </map>
    <sample>
        <numComponents>7</numComponents>
        <name>Nucleosome Remodeling and Deacetylase Complex</name>
        <details>Purified endogenously from MEL cells.</details>
        <sampleComponentList>
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                <entry>protein</entry>
                <sciName>Nucleosome Remodeling and Deacetylase Complex</sciName>
                <molWtExp units="MDa">0.8</molWtExp>
                <details>Purified endogenously from MEL cells.</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource />
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                <entry>protein</entry>
                <sciName>Metastasis-associated protein MTA2</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
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                <entry>protein</entry>
                <sciName>Histone deacetylase 1</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>protein</entry>
                <sciName>Histone-binding protein RBBP4</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="5">
                <entry>protein</entry>
                <sciName>Methyl-CpG-binding protein MBD3</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="6">
                <entry>protein</entry>
                <sciName>GATA zinc finger domain-containing protein 2A</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
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            </sampleComponent>
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                <entry>protein</entry>
                <sciName>Chromodomain-helicase-DNA-binding protein 4</sciName>
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                    <sciSpeciesName ncbiTaxId="10090">Mus musculus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
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            </sampleComponent>
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            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>OTHER</detector>
            <microscope>FEI TECNAI 12</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">120</acceleratingVoltage>
        </imaging>
        <imageAcquisition>
            <numDigitalImages>1332</numDigitalImages>
        </imageAcquisition>
        <specimenPreparation>
            <specimenState>particle</specimenState>
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            <buffer>
                <ph>7.5</ph>
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        </specimenPreparation>
    </experiment>
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        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>33.0</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
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            <appliedSymmetry>C1</appliedSymmetry>
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