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    <admin>
        <lastUpdate>2021-02-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2017-07-25</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2021-02-10</headerReleaseDate>
        <obsoletedDate>2021-02-10</obsoletedDate>
        <title>Emptied bacterophage phiX174 complexed with lipopolysaccharides</title>
        <authors>Rossmann MG, Sun Y, Klose T, Roznowski A, Fane BA, Pollack L, Tokuda J, Mauney A</authors>
        <primaryReference published="true">
            <journalArticle>
                <authors>Sun L, Young LN, Zhang X, Boudko SP, Fokine A, Zbornik E, Roznowski AP, Molineux IJ, Rossmann MG, Fane BA</authors>
                <articleTitle>Icosahedral bacteriophage phiX174 forms a tail for DNA transport during infection.</articleTitle>
                <journal>Nature</journal>
                <volume>505</volume>
                <firstPage>432</firstPage>
                <lastPage>435</lastPage>
                <year>2014</year>
                <externalReference type="pubmed">24336205</externalReference>
                <externalReference type="doi">doi:10.1038/nature12816</externalReference>
                <externalReference type="issn">1476-4687</externalReference>
                <externalReference type="csd">0006</externalReference>
                <externalReference type="astm">NATUAS</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>160</numRows>
            <numColumns>160</numColumns>
            <numSections>160</numSections>
        </dimensions>
        <origin>
            <originRow>-80</originRow>
            <originCol>-80</originCol>
            <originSec>-80</originSec>
        </origin>
        <limit>
            <limitRow>79</limitRow>
            <limitCol>79</limitCol>
            <limitSec>79</limitSec>
        </limit>
        <spacing>
            <spacingRow>160</spacingRow>
            <spacingCol>160</spacingCol>
            <spacingSec>160</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">518.4</cellA>
            <cellB units="A">518.4</cellB>
            <cellC units="A">518.4</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.043593593</minimum>
            <maximum>0.07500575</maximum>
            <average>-0.00037763966</average>
            <std>0.009705934</std>
        </statistics>
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        <details />
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            <pixelZ units="A">3.2400002</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.028</contourLevel>
        <annotationDetails>Emptied bacteriophage phix174 complexed with lipopolysaccharide bilayer disk, low pass-filtered to 8 Angstrom</annotationDetails>
    </map>
    <sample>
        <numComponents>2</numComponents>
        <name>emptied bacteriophage phiX174 complexed with lipopolysacchrides from Salmonella enterica serotype typhimurium TV119 (Ra mutant)</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>emptied bacteriophage phiX174 complexed with lipopolysacchrides from Salmonella enterica serotype typhimurium TV119 (Ra mutant)</sciName>
                <molWtTheo units="MDa">0.01</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="1246486">synthetic Enterobacteria phage phiX174.1f</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource />
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>cellular-component</entry>
                <sciName>lipopolysaccharides (rough strains) from Salmonella enterica serotype typhimurium TV119 (Ra mutant)</sciName>
                <cellular-component>
                    <sciSpeciesName ncbiTaxId="28901">Salmonella enterica</sciSpeciesName>
                    <sciSpeciesStrain>typhimurium TV119</sciSpeciesStrain>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <natSource>
                        <cellLocation>outer membrane</cellLocation>
                    </natSource>
                </cellular-component>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>80</humidity>
            <temperature units="Kelvin">298</temperature>
            <instrument>GATAN CRYOPLUNGE 3</instrument>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">30.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>GATAN K2 SUMMIT (4k x 4k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">1</specimenConc>
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                <ph>7.4</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <algorithm>BACK PROJECTION</algorithm>
            <software>RELION</software>
            <resolutionByAuthor>9.9</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C5</appliedSymmetry>
            <numProjections>2400</numProjections>
        </singleParticle>
    </processing>
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