2021-02-01 OBS RCSB RCSB RCSB 2017-07-25 2021-02-10 2021-02-10 2021-02-01 Emptied bacterophage phiX174 complexed with lipopolysaccharides Rossmann MG Sun Y Klose T Roznowski A Fane BA Pollack L Tokuda J Mauney A Sun L Young LN Zhang X Boudko SP Fokine A Zbornik E Roznowski AP Molineux IJ Rossmann MG Fane BA Icosahedral bacteriophage phiX174 forms a tail for DNA transport during infection. Nature UK 505 432 435 2014 24336205 doi:10.1038/nature12816 1476-4687 0006 NATUAS EMD-8858 associated EM volume EMD-8862 other EM volume emptied bacteriophage phiX174 complexed with lipopolysacchrides from Salmonella enterica serotype typhimurium TV119 (Ra mutant) emptied bacteriophage phiX174 complexed with lipopolysacchrides from Salmonella enterica serotype typhimurium TV119 (Ra mutant) 0 synthetic Enterobacteria phage phiX174.1f 0.01 lipopolysaccharides (rough strains) from Salmonella enterica serotype typhimurium TV119 (Ra mutant) 1 Salmonella enterica typhimurium TV119 outer membrane singleParticle particle 1 7.4 ETHANE 80 298 GATAN CRYOPLUNGE 3
Mixed with LPS and incubated at 33 degrees C for 1 minute
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 100.0 2.7 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) COUNTING 30.0 1 7500 CTFFIND3
An icosahedral reconstruction from a small dataset
 1 C5 BACK PROJECTION 9.9 FSC 0.143 CUT-OFF RELION 1.4 2400 PROJECTION MATCHING RELION PROJECTION MATCHING 4 emd_8858.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 -80 -80 -80 160 160 160 518.4 518.4 518.4 90.0 90.0 90.0 X Y Z -0.043593593 0.07500575 -0.00037763966 0.009705934 3.2400002 3.2400002 3.2400002 0.028 AUTHOR Emptied bacteriophage phix174 complexed with lipopolysaccharide bilayer disk, low pass-filtered to 8 Angstrom emd_8858_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 -80 -80 -80 160 160 160 518.4 518.4 518.4 90.0 90.0 90.0 X Y Z -0.067826636 0.11293771 0.00097474846 0.011703841 3.2400002 3.2400002 3.2400002 AUTHOR Unprocessed half map #1 from Relion Refine3D emd_8858_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 160 160 160 -80 -80 -80 160 160 160 518.4 518.4 518.4 90.0 90.0 90.0 X Y Z -0.079552844 0.11241061 0.0009114844 0.011767034 3.2400002 3.2400002 3.2400002 AUTHOR Unprocessed half map #2 from Relion Refine3D