2024-10-09 OBS RCSB RCSB RCSB 2017-07-17 2017-08-09 2017-10-11 2024-10-09 2024-10-09 National Institutes of Health/National Cancer Institute R01CA193578-01A1 United States Mutated BRCA1-BARD1 Kelly DF Dearnaley WJ Breast Cancer Susceptibility Protein, DNA Damage Repair, DNA BINDING PROTEIN Thorp HH Editorial Retraction: Structural analysis of BRCA1 reveals modification hotspot Sci Adv US 10 2024 doi:10.1126/sciadv.ads9451 2375-2548 Liang Y Dearnaley WJ Varano AC Winton CE Gilmore BL Alden NA Sheng Z Kelly DF Structural analysis of BRCA1 reveals modification hotspot. Sci Adv US 3 2017 28948225 doi:10.1126/sciadv.1701386 2375-2548 EMD-8834 other EM volume Mutated BRCA1-BARD1 complex. Mutated BRCA1-BARD1 complex. 0 Homo sapiens singleParticle particle 0.02 7.2 HEPES buffer
20 mM HEPES buffer pH 7.2, 150 mM NaCl, 10 mM CaCl2, 10 mM MgCl2
 NEGATIVE Uranyl Formate
1% uranyl formate
 Ted Pella COPPER 400 CARBON CONTINUOUS GLOW DISCHARGE AIR FEI TECNAI SPIRIT FLOOD BEAM BRIGHT FIELD LAB6 120 2.0 -1.5 68000.0 SIDE ENTRY, EUCENTRIC FEI EAGLE (2k x 2k) 4 100 5.0 1 5000 1 C1 14.7 FSC 0.5 CUT-OFF RELION 1.3b 4222 OTHER RELION 1.3b
Empirical Bayesian approach
 OTHER RELION 1.3b
Empirical Bayesian approach
 1 4222.0 RELION 1.3b emd_8833.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 60 60 60 45 45 45 60 60 60 264.0 264.0 264.0 90.0 90.0 90.0 X Y Z -0.051657803 0.09341081 0.0011541239 0.0135132745 4.4 4.4 4.4 0.0589 AUTHOR Model of BRCA1-BARD1 isolated from the mutated human breast cancer cell line HCC1937