2018-02-14 OBS 2016-11-04 RCSB RCSB 2017-03-15 2018-02-07 2018-02-14 BK channel with membrane density subtracted 8.1 angstrom (hSlo protein was reconstituted into POPC liposomes) Tonggu L, Zhan X, Wang L Tonggu L, Zhan X, Wang L Cryo-EM study of the BK potassium channel in lipid membranes using random spherically constrained single-particle (RSC) method To Be Published 0353 emd_8468.map.gz Image stored as Reals 96 96 96 0.0 0.0 0.0 95.0 95.0 95.0 96 96 96 241.92 241.92 241.92 90.0 90.0 90.0 X Y Z -0.005202601 0.008175316 -0.000054849264 0.0005461847 1
2.52 2.52 2.52 0.000706 BK channel with membrane density subtracted 8.1 angstrom 1 large conductance voltage- and calcium-activated potassium (BK) channels
full-length human Slo protein (GI:507922)
protein large conductance voltage- and calcium-activated potassium (BK) channels 500
full-length human Slo protein (GI:507922)
Homo sapiens false Homo sapiens hek293 pcdna3.1 ETHANE 40 298 HOMEMADE PLUNGER
Two-dimensional (2D) streptavidin crystals were grown at room temperature on perforated carbon grids. After the 2D streptavidin crystal was washed with crystal buffer (150mM NaCl, 50mM HEPES, pH 7.3) once and buffer A (20 mM HEPES, pH 7.2, 150 mM KC) twice, the crystal was incubated with BK proteoliposome suspensions for 10-40 min to allow binding. The sample was blotted at room temperature and immediately fast-frozen in liquid ethane cooled by liquid nitrogen..
FIELD EMISSION GUN 1.0 BRIGHT FIELD FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) 2.0 FEI TECNAI F20 GATAN LIQUID NITROGEN 200 particle 0.2 7.2 hexane wash singleParticle FOURIER SPACE 8.1 FSC 0.143 CUT-OFF RSC method written in matlab C4 88041