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<emdEntry accessCode="8426" version="1.9.6">
    <admin>
        <lastUpdate>2017-07-27</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2016-10-06</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2016-10-19</headerReleaseDate>
        <obsoletedDate>2016-10-19</obsoletedDate>
        <title>Initial contact of HIV-1 Env with CD4: cryo-EM structure of BG505 DS-SOSIP trimer in complex with CD4 and antibody PGT145</title>
        <authors>Acharya P, Kwong PD, Potter CS, Carragher B</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Liu Q, Acharya P, Dolan MA, Carragher B, Potter CS, Kwong PD, Lusso P</authors>
                <articleTitle>Structure and functional characterization of the initial quaternary contact of CD4 with the HIV-1 envelope trimer</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
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            <numRows>320</numRows>
            <numColumns>320</numColumns>
            <numSections>320</numSections>
        </dimensions>
        <origin>
            <originRow>0.0</originRow>
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        <limit>
            <limitRow>319.0</limitRow>
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        <spacing>
            <spacingRow>320</spacingRow>
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        </spacing>
        <cell>
            <cellA units="A">352.0</cellA>
            <cellB units="A">352.0</cellB>
            <cellC units="A">352.0</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
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            <maximum>0.046937425</maximum>
            <average>0.00023340447</average>
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        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details></details>
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        </pixelSpacing>
        <contourLevel source="author">0.011</contourLevel>
    </map>
    <sample>
        <numComponents>5</numComponents>
        <name>HIV-1 Env trimer</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>HIV-1 Env trimer</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="11676">Human immunodeficiency virus 1</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                        <vector>pVRC8400</vector>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>HIV-1 Env trimer in complex with antibody PGT145 and CD4</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="11676">Human immunodeficiency virus 1</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource/>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>Antibody PGT145</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9606">Homo sapiens</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                        <vector>pVRC8400</vector>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>protein</entry>
                <sciName>CD4</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9606">Homo sapiens</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                        <vector>pVRC8400</vector>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="5">
                <entry>protein</entry>
                <sciName>HIV-1 trimer</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="11676">Human immunodeficiency virus 1</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                        <vector>pVRC8400</vector>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
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    <experiment>
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            <cryogenName>ETHANE</cryogenName>
            <humidity>85</humidity>
            <temperature units="Kelvin">298</temperature>
            <instrument>GATAN CRYOPLUNGE 3</instrument>
            <details></details>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">8.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>GATAN K2 SUMMIT (4k x 4k)</detector>
            <calibratedMagnification>22000.0</calibratedMagnification>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition/>
        <fitting>
            <pdbEntryIdList/>
            <refProtocol>rigid body</refProtocol>
            <targetCriteria>Correlation Coefficient</targetCriteria>
            <refSpace>REAL</refSpace>
        </fitting>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">1</specimenConc>
            <buffer>
                <ph>7.2</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>7.5</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <numProjections>48364</numProjections>
        </singleParticle>
    </processing>
</emdEntry>
