2021-02-01 OBS RCSB RCSB RCSB 2018-05-20 2021-02-10 2021-02-10 2021-02-01 National Institutes of Health/National Institute Of Allergy and Infectious Diseases UM1 AI100663 United States Bill & Melinda Gates Foundation OPP1084519 United States Bill & Melinda Gates Foundation OPP1115782 United States BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP43 fragment antigen binding Ozorowski G Steichen JM Schief WR Ward AB Steichen JM Ozorowski G Ward AB Schief WR HIV vaccine design to initiate induction of rare HCDR3-dependent antibodies To Be Published 0353 EMD-7886 associated EM volume
BG505 MD39 SOSIP trimer in complex with mature BG18 fragment antigen binding
 EMD-7885 other EM volume EMD-7884 other EM volume BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP43 fragment antigen binding BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP43 fragment antigen binding 0 1 2 3 0.57 SOSIP trimer 1 1 Human immunodeficiency virus 1 Homo sapiens HEK293F HMP43 fragment 1 2 3 Homo sapiens Homo sapiens HEK293 BG505 MD64 N332-GT5 SOSIP Human immunodeficiency virus 1 Homo sapiens LEVO AENLWVTVYYGVPVWKDAETTLFCASDAKAYETEKHNVWATHACVPTDPNPQEIHLENVTEEFNMWKNNMVEQMHEDIISLWDQSLKPCVKLTPLCVTLQCTNYAPKLRSMMRGEIKNCSFNMTTELRDKKQKVYSLFYRLDVVQINENQGNRSNNSNKEYRLINCNTSAITQACPKVSFEPIPIHYCAPAGFAILKCKDKKFNGTGPCPSVSTVQCTHGIKPVVSTQLLLNGSLAEEEVIIRSENITNNAKNILVQLNTPVQINCTRPSNNTVKSIRIGPGQAFYYFGDVLGHVRMAHCNISKATWNETLGKVVKQLRKHFGNNTIIRFAQSSGGDLEVTTHSFNCGGEFFYCNTSGLFNSTWISNTSVQGSNSTGSNDSLILPCWIKQIINMWQRIGQAMYAPPIQGVIRCVSNITGLILTRDGGSTNSTTETFRPGGGDMRDNWRSELYKYKVVKIEPLGVAPTRCKRRVVGRRRRRRAVGIGAVSLGFLGAAGSTMGAASMTLTVQARNLLSGIVQQQSNLLRAPEPQQHLLKDTHWGIKQLQARVLAVEHYLRDQQLLGIWGCSGKLICCTNVPWNSSWSNRNLSEIWDNMTWLQWDKEISNYTQIIYGLLEESQNQQEKNEQDLLALDGTKHHHHHH BG18-like precursor HMP43 fragment antigen binding heavy chain Homo sapiens Homo sapiens LEVO EVQLVESGGGLVQPGRSLRLSCAASGFTFDDYAMHWVRQAPGKGLEWVSGISWNSGSIGYADSVKGRFTISRDNAKNSLYLQMNSLRAEDTALYYCAKDIESRYFDWDNYYYYGMDVWGKGTTVTVSS BG18-like precursor HMP43 fragment antigen binding light chain Homo sapiens Homo sapiens LEVO SYELTQPPSVSVSPGQTASITCSGDKLGDKYACWYQQKPGQSPVLVIYQDSKRPSGIPERFSGSNSGNTATLTISGTQAMDEADYYCQAWDSSVVFGGGTKLTVL singleParticle particle 3 7.4 50.0 Tris 150.0 NaCl sodium chloride 0.005 Lauryl Maltose Neopentyl Glycol Quantifoil R1.2/1.3 COPPER 400 CARBON HOLEY PLASMA CLEANING OTHER
Gatan Model 950 Advanced Plasma System
 ETHANE 100 283 FEI VITROBOT MARK IV
8 seconds blotting time.
FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 100.0 2.7 1.0 2.5 73000.0 GATAN 914 HIGH TILT LIQUID NITROGEN CRYO TRANSFER TOMOGRAPHY HOLDER NITROGEN FEI CETA (4k x 4k) 4096 4096 1.0 50.0 1 Gctf 1.06 4ZMJ
PDB coordinates converted to low pass filtered map
 C3 20.0 FSC 0.143 CUT-OFF RELION 2.1 3619 PROJECTION MATCHING RELION 2.1 PROJECTION MATCHING RELION 2.1 emd_7886.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 380.16 380.16 380.16 90.0 90.0 90.0 X Y Z -0.06916224 0.27754518 0.0010606298 0.020397883 1.98 1.98 1.98 0.06 AUTHOR sharpened map emd_7886_msk_1.map emd_7886_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 380.16 380.16 380.16 90.0 90.0 90.0 X Y Z -0.039204974 0.13739693 0.00051118806 0.010071941 1.98 1.98 1.98 AUTHOR half map 1 emd_7886_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 380.16 380.16 380.16 90.0 90.0 90.0 X Y Z -0.033134196 0.13233756 0.0005494417 0.010075598 1.98 1.98 1.98 AUTHOR half map 2