<?xml version='1.0' encoding='utf-8'?>
<emdEntry accessCode="7886" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2021-02-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-05-20</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2021-02-10</headerReleaseDate>
        <obsoletedDate>2021-02-10</obsoletedDate>
        <title>BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP43 fragment antigen binding</title>
        <authors>Ozorowski G, Steichen JM, Schief WR, Ward AB</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Steichen JM, Ozorowski G, Ward AB, Schief WR</authors>
                <articleTitle>HIV vaccine design to initiate induction of rare HCDR3-dependent antibodies</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="28312" type="map">emd_7886.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>192</numRows>
            <numColumns>192</numColumns>
            <numSections>192</numSections>
        </dimensions>
        <origin>
            <originRow>0</originRow>
            <originCol>0</originCol>
            <originSec>0</originSec>
        </origin>
        <limit>
            <limitRow>191</limitRow>
            <limitCol>191</limitCol>
            <limitSec>191</limitSec>
        </limit>
        <spacing>
            <spacingRow>192</spacingRow>
            <spacingCol>192</spacingCol>
            <spacingSec>192</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">380.16</cellA>
            <cellB units="A">380.16</cellB>
            <cellC units="A">380.16</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.06916224</minimum>
            <maximum>0.27754518</maximum>
            <average>0.0010606298</average>
            <std>0.020397883</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">1.98</pixelX>
            <pixelY units="A">1.98</pixelY>
            <pixelZ units="A">1.98</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.06</contourLevel>
        <annotationDetails>sharpened map</annotationDetails>
    </map>
    <sample>
        <numComponents>6</numComponents>
        <name>BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP43 fragment antigen binding</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>BG505 MD64 N332-GT5 SOSIP trimer in complex with BG18-like precursor HMP43 fragment antigen binding</sciName>
                <molWtTheo units="MDa">0.57</molWtTheo>
                <protein>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource />
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>SOSIP trimer</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="11676">Human immunodeficiency virus 1</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                        <expSystemStrain>HEK293F</expSystemStrain>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>HMP43 fragment</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9606">Homo sapiens</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                        <expSystemStrain>HEK293</expSystemStrain>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>protein</entry>
                <sciName>BG505 MD64 N332-GT5 SOSIP</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="11676">Human immunodeficiency virus 1</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="5">
                <entry>protein</entry>
                <sciName>BG18-like precursor HMP43 fragment antigen binding heavy chain</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9606">Homo sapiens</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="6">
                <entry>protein</entry>
                <sciName>BG18-like precursor HMP43 fragment antigen binding light chain</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9606">Homo sapiens</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="9606">Homo sapiens</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>100</humidity>
            <temperature units="Kelvin">283</temperature>
            <instrument>FEI VITROBOT MARK IV</instrument>
            <details>8 seconds blotting time. </details>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">50.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <nominalDefocusMin units="nm">1000.0</nominalDefocusMin>
            <nominalDefocusMax units="nm">2500.0</nominalDefocusMax>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI CETA (4k x 4k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <microscope>FEI TALOS ARCTICA</microscope>
            <specimenHolderModel>GATAN LIQUID NITROGEN</specimenHolderModel>
            <acceleratingVoltage units="kV">200</acceleratingVoltage>
            <nominalMagnification>73000</nominalMagnification>
        </imaging>
        <imageAcquisition />
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">3</specimenConc>
            <buffer>
                <ph>7.4</ph>
            </buffer>
            <specimenSupportDetails>Gatan Model 950 Advanced Plasma System</specimenSupportDetails>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>20.0</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C3</appliedSymmetry>
            <numProjections>3619</numProjections>
        </singleParticle>
    </processing>
</emdEntry>