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<emdEntry accessCode="7842" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2021-02-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-05-01</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2021-02-10</headerReleaseDate>
        <obsoletedDate>2021-02-10</obsoletedDate>
        <title>BBSome complex</title>
        <authors>Chou H, Apelt L, Farrell DP, White SR, Woodsmith J, Svetlov V, Goldstein JS, Nager AR, Li Z, Muller J, Dollfus H, Nudler E, Stelzl U, DiMaio F, Walz T, Nachury NV</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Chou H, Apelt L, Farrell DP, White SR, Woodsmith J, Svetlov V, Goldstein JS, Nager AR, Li Z, Muller J, Dollfus H, Nudler E, Stelzl U, DiMaio F, Walz T, Nachury MV</authors>
                <articleTitle>The molecular architecture of the BBSome and its implications for facilitated transition zone crossing</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="131073" type="map">emd_7842.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>320</numRows>
            <numColumns>320</numColumns>
            <numSections>320</numSections>
        </dimensions>
        <origin>
            <originRow>-160</originRow>
            <originCol>-160</originCol>
            <originSec>-160</originSec>
        </origin>
        <limit>
            <limitRow>159</limitRow>
            <limitCol>159</limitCol>
            <limitSec>159</limitSec>
        </limit>
        <spacing>
            <spacingRow>320</spacingRow>
            <spacingCol>320</spacingCol>
            <spacingSec>320</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">393.6</cellA>
            <cellB units="A">393.6</cellB>
            <cellC units="A">393.6</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.0065787276</minimum>
            <maximum>0.02200084</maximum>
            <average>0.00013429897</average>
            <std>0.0014194305</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">1.23</pixelX>
            <pixelY units="A">1.23</pixelY>
            <pixelZ units="A">1.23</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.0083</contourLevel>
        <annotationDetails>primary map</annotationDetails>
    </map>
    <sample>
        <numComponents>9</numComponents>
        <name>BBSome</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>BBSome</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource />
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>BBS5</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>BBS9</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>protein</entry>
                <sciName>BBS1</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="5">
                <entry>protein</entry>
                <sciName>BBS7</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="6">
                <entry>protein</entry>
                <sciName>BBS8</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="7">
                <entry>protein</entry>
                <sciName>BBS18</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="8">
                <entry>protein</entry>
                <sciName>BBS4</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="9">
                <entry>protein</entry>
                <sciName>BBS2</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="9913">Bos taurus</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">89.7</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>GATAN K2 SUMMIT (4k x 4k)</detector>
            <microscope>FEI POLARA 300</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition>
            <numDigitalImages>870</numDigitalImages>
        </imageAcquisition>
        <imageAcquisition>
            <numDigitalImages>1630</numDigitalImages>
        </imageAcquisition>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>7</ph>
                <details>40 mM HEPES, 220 mM NaCl, 1 mM DTT</details>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>4.9</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C1</appliedSymmetry>
            <numProjections>179280</numProjections>
        </singleParticle>
    </processing>
</emdEntry>