2021-02-01 OBS RCSB RCSB RCSB 2018-05-01 2021-02-10 2021-02-10 2021-02-01 BBSome complex Chou H Apelt L Farrell DP White SR Woodsmith J Svetlov V Goldstein JS Nager AR Li Z Muller J Dollfus H Nudler E Stelzl U DiMaio F Walz T Nachury NV Chou H Apelt L Farrell DP White SR Woodsmith J Svetlov V Goldstein JS Nager AR Li Z Muller J Dollfus H Nudler E Stelzl U DiMaio F Walz T Nachury MV The molecular architecture of the BBSome and its implications for facilitated transition zone crossing To Be Published 0353 EMD-7840 associated EM volume BBSome BBSome 0 1 2 3 4 5 6 7 8 Bos taurus BBS5 Bos taurus LEVO MSVLDALWEDRDVRFDVSSQQMKTRPGEVLIDCLDSVEDTKGNNGDRGRLLVTNLRIVWH SLALPRVNLSIGYNCILNITTRTANSKLRGQTEALYVLTKCNSTRFEFIFTNLVPGSPRL YTSLIAVHRAYETSKMYRDFKLRSALIQNKQLRLLPQENVYNKINGVWNLSSDQGNLGTF FITNVRIVWHANMNDSFNVSIPYLQIRSVKIRDSKFGLALVIESSQQSGGYVLGFKIDPV EKLQESVKEINSLHKVYSANPIFGVDYEMEEKPQPLEALTVKQIQDDVEIDSDDHTDAFV AYFADGNKQQDREPVFSEELGLAIEKLKDGFTLQGLWEVMN BBS9 Bos taurus LEVO MSLFKARDWWSTVLGDKEEFDQGCLCLADVDNTGNGQDKIIVGSFMGYLRIFNPHPVKTG DGAQAEDLLLEVHLRDPILQVEVGKFVSGTEMLHLAVLHSRKLCVYSVSGTLGNVEHGNQ YQIKLMYEHNLQRTACNMTYGSFGGVKGRDLICIQSVDGMLMVFEQESYAFGRFLPGSLL PGPLAYSSRTDSFITVSSCHQVESYKYQVLAFATDADKRQETEQQKHGSGKRLVVDWTLN IGEQAIDICIVSFIQSASSVFVLGERNFFCLKDNGQIQFMKKLDYSPSCFLPYCSVSEGT INTLIGNHNNMLHIYQDVTLKWATQLPHVPVAVRVGCLHDLKGVIVTLSDDGHLQCSYLG TDPSLFQAPKVESRELNYDELDMELKELQKVIKNVNKSQDVWPLTEREDDLKVSAMVSPN FDSVSQATDVEVGADLVPSVTVKVTLKNRVALQKIKLSIYVQPPLVLTGDQFTFEFMAPE MTRTVGFSVYLKGSYSPPELEGNAVVSYSRPTERNPDGIPRVSQCKFRLPLKLVCLPGQP SKTASHKLTIDTNKSPVSLLSLFPGFAKQSEDDQVNVMGFRFLGGSQVTLLASKTSQRYR IQSEQFEDLWLITNELIIRLQEYFEKQGIKDFTCSFSGSVPLEEYFELIDHHFELRINGE KLEELLSERAVQFRAIQRRLLTRFKDKTPAPLQHLDTLLDGTYKQVIALADAVEENQDNL FQSFTRLKSATHLVILLIGLWQKLSADQIAILEAAFLPLQQDTQELGWEETVDAALSHLL KTCLSKSSKEQALNLNSQLGIPKDTSQLKKHITLFCDRLAKGGRLCLSTDAAAPQTMVMP GGCATIPESDLEGRSIDQDSSELFTNHKHLMVETPVPEVSPLQGVTE BBS1 Bos taurus LEVO MAATSSSDSDGGKGESSEANSKWLDSLSDSMANIHTFSACLALADFHGDGEYKLAMGDLG PDGRQPRLKVLKGHTLVSQKPLPDLPAAAVTFLMASHEPRTPALAIASGPCVYVYKNLKP YFKFSLPSLPTNPLEQDLWNQAKEDQIDPLTLKEMLEGIREKAEVPLSVQSLRFLPLELS EMEAFVNQHKSKSIRRQTVITTMTTLKKNLADEDAVSCLVLGTENKELLVLDPEAFTILA KMSLPSVPAFLEASGQFDVEFRLAAACRNGSIYILRRDSKRPKYCIELGAQPVGLVGVHK VLVVGSNQDSLHGFTYKGKRLWTVQMPAAILAMNLLEQHSRGLQAVMAALANEEVRIYHD KVLLNVIRTPEAVTSLCFGRYGREDNTLIMTTLGGGLIIKILKRTAVFAEGGGEAGPPPS QAIKLNVPRKTRLYVDQTLREREAGTAMHRTFQADLYLLRLRAARAYVQALESSLSPVSL TAREPLKLHAVVQGLGPTFKLTLHLQNTSTARPILGLVVCFLYNEVLYALPRAFFKVPLL VPGLNYPLETFVKSLSDKGISDIIKVLVLREGQSTPLLSAHINMPMSEGLAAD BBS7 Bos taurus LEVO MLFSSSVPFVGVTSQKTMKLLPASKHRATQKVVVGDHDGIVMCFGMKKGEAVTVFKTLPG QKIARLELGGALNTPQEKIFIAAGSEIRGFTKRGKQFLSFETNLTESIKAMHISGSDLFL SASYIYNHYCDCKDQHYYLSGDKINDVICLPVERLLREVPVLACQDRVLRVLQGSDVTYE IEVPGPPTVLALHNGNGGDSGEDLLFGTSDGKLGLIQITTSKPIHKWEIRNEKKRGGILC VDSFDIVGDGVKDLLVGRDDGMVEVYGFDNANEPVLRFDHTLSESVTSIQGGCVGKDGYD EIVVSTYSGWITGLTTEPVHKESGPGEELKFNQEMQNKISSLRSELEQLQYKVLQEREKY QQSSQSSKAKSAVPSFSVNDKFTLNKDDASYSLILEVQTAIDNVLIQSDVPIDLLDVDKN SAVVSFSSCDSESNDNFLLATYRCQANTTRLELKIRSIEGQYGTLQAYVTPRIQPKTCQV RQYHIKPLSLHQRTHFIDHDRPMNTLTLTGQFSFSELHSWVVFCMPEVPEKPPAGECVTF YFQNTFLDTQLESTYRKGEGVFKSDNISTISILKDVLSKEATKRKINLNISYEINEVSVK HTLKLIHPKLEYQLLLAKKVQLIDALKELQVHEGNTNFLIPEYRCILEEADHLQEEYKKQ PAHLERLYGMITDLFIDKFKFKGTNVKTKVPLLLEILDSYDQNALIAFFDAA BBS8 Bos taurus LEVO APASDMEPLLLAWSYFRRRRFQLCADLCTQMLEKSPCDQAAWILKARALTEMVYVDEIDV DEEGIAEMILDENAIAQVPRPGTSLKLPGTNQTGGPSPAVRPVTQAGRPITGFLRPSTQS GRPGTIEQAIKTPRTAYTARPIASSSGRFVRLGTASMLTSPDGPFINLSRLNLAKYAQKP KLAKALFEYIFHHENDVKTALDLAALSTEHSQYKDWWWKVQIGKCYYRLGLYREAEKQFK SALKQQEMVDTFLYLAKVYISLDQPLTALNLFKQGLDKFPGEVTLLCGIARIYEEMNNIS SATEYYKEVLKQDNTHVEAIACIGSNHFYTDQPEVALRFYRRLLQMGVYNCQLFNNLGLC CFYAQQYDMTLTSFERALSLAENEEEVADVWYNLGHVAVGTGDTNLAHQCFRLALVSNNQ HAEAYNNLAVLEMRRGHVEQAKALLQTASSLAPHMYEPHFNFATISDKIGDLQRSYAAAK KSEAAFPDHVDTQHLIKQLEQHFAML BBS18 Bos taurus LEVO MLSAAHKSFCFPSATQDETVSNISDMAETKSMFREVLPKQDLGQLYVEDITTMVLCKPKL LPLKSLTLEKLEKMQQAAQDTIHQQEMTEKEQKITH BBS4 Bos taurus LEVO MAEEKLSARTQLPVSAESQKPVLKKAPEFPILEKQNWLIHLYYIQKDYEACKAVIKEQLQ ETHGLCEYAIYVQALIFRLEGNIQESLRLFQMCAFLSPQCADNLKQVARSLFLLGKHKAA IEVYNEAAKLNQKDWEICHNLGVCYIYLKQFDKAQDQLHNALHLNRHDLTYIMLGKIFLL KGDLDKAIEIYKKAVEFSPENTELLTTLGLLYLQLGIYQKAFEHLGNTLTYDPTNYKAIL AAGSMMQTHGDFDVALTKYKVVACAVIESPPLWNNIGMCFFGKKKYVAAISCLKRANYLA PLDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNLEDSENAKRA YEEAVRLDKCNPLVNLNYAVLLYNQGEKRDALAQYQEMEKKVNLLKYSSSLEFDPEMVEV AQKLGAALQVGEALVWTKPVKDPKSKHQTASTSKAAGFQQPLGSNQALGQAMSSAATCRK LSSGAGGTSQLTKPPSLPLEPEPTVEAQPTEASAQTREK BBS2 Bos taurus LEVO MLQPVFTLKLRHKISPRMVAVGRYDGTHPCLAAATQAGKVFIHNPHSRSQHLGAPRVLQS PLESDVSLLNINQTVSCLTAGVLNPELGYDALLVGTQTNLLAYDVYNNSDLFYREVADGA SAIVLGTLGDITSPLAIIGGNCALQGFNHEGNDLFWTVTGDNVHSLALCDFDGDGKKELL VGSEDFDIRVFKEDEIVAEMSETEIITSLCPMYGSRFGYALSNGTVGVYDKTARYWRIKS KNQAMSIHAFDLNSDGVCELITGWSNGKVDARSDRTGEVIFKDNFSSAIAGVVEGDYRME GCQQLICCSVDGEIRGYLPGTAEMRGNLMDISVEQDLIRELSQKKQNLLLELRNYEENAK AELSSPLNEADGHRGVIPANTKHHTALSVSLGSEAQAAHAELCISTSNDTIIRAVLIFAE GVFAGESHVVHPSVHHLSSSVRIPITPPKDIPVDLHLKTFVGYRSSTQFHVFELTRQLPR FSMYALTSPDPASEPLSYVNFIIAERAQRVVMWLNQNFLLPEDTNIQNAPFQVCFTSLRN GGQLYIKIKLSGEITVNTDDIDLAGDIIQSMASFFAIEDLQVEADFPVYFEELRKVLVKV DEYHSVHQKLSADMADNSNLIRSLLVQAEDARLMRDMKTMKNRYKELYDLNKDLLNGYKI RCNNHTELLGSLKAVNQAIQRAGHLRVGKPKNQVITACRDAIRSNNINMLFRIMRVGTAS S singleParticle particle 7.0
40 mM HEPES, 220 mM NaCl, 1 mM DTT
 Quantifoil R1.2/1.3 COPPER 400 GLOW DISCHARGE ETHANE FEI POLARA 300 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 870 46.8 GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 1630 89.7 1 CTFFIND C1 4.9 FSC 0.143 CUT-OFF RELION 1.3 33492 PROJECTION MATCHING RELION 1.3 PROJECTION MATCHING RELION 1.3 RELION 1.3 emd_7840.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 393.6 393.6 393.6 90.0 90.0 90.0 X Y Z -0.025044426 0.09222394 0.0002497337 0.0033914752 1.23 1.23 1.23 0.03 AUTHOR primary map