2021-02-01 OBS RCSB RCSB RCSB 2018-04-30 2021-02-10 2021-02-10 2021-02-01 Spinach chloroplast ATP synthase (reduced) Yang J-H Williams D Fromme P Chiu P-L Yang J-H Williams D Fromme P Chiu P-L Mechanism of light sensitivity of chloroplast ATP synthase revealed by single-particle cryo-EM To Be Published 0353 EMD-7838 associated EM volume
Spinach chloroplast ATP synthase (reduced)
 6d9v FULLOVERLAP Chloroplast ATP synthase complex Chloroplast ATP synthase complex 0 1 2 3 4 5 6 7 8 9
Spinach chloroplast ATP synthase from thylakoid membranes
Spinacia oleracea Leaves Chloroplast Thylakoid membranes 0.594 ATP synthase subunit alpha, chloroplastic Spinach 0.055505199 3 LEVO MATIRADEISKIIRERIEGYNREVKVVNTGTVLQVGDGIARIHGLDEVMAGELVEFEEGTIGIALNLESNNVGVVLMGDG LMIQEGSSVKATGRIAQIPVSEAYLGRVINALAKPIDGRGEITASESRLIESPAPGIMSRRSVYEPLQTGLIAIDAMIPV GRGQRELIIGDRQTGKTAVATDTILNQQGQNVICVYVAIGQKASSVAQVVTNFQERGAMEYTIVVAETADSPATLQYLAP YTGAALAEYFMYRERHTLIIYDDLSKQAQAYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKLSSLLGEGSMTALPIV ETQAGDVSAYIPTNVISITDGQIFLSADLFNAGIRPAINVGISVSRVGSAAQIKAMKKVAGKLKLELAQFAELEAFAQFA SDLDKATQNQLARGQRLRELLKQPQSAPLTVEEQVMTIYTGTNGYLDSLELDQVRKYLVELRTYVKTNKPEFQEIISSTK TFTEEAEALLKEAIQEQMERFLLQEQA 3.6.3.14 ATP synthase subunit beta, chloroplastic Spinach 0.053797367 3 LEVO MRINPTTSDPGVSTLEKKNLGRIAQIIGPVLDVAFPPGKMPNIYNALIVKGRDTAGQPMNVTCEVQQLLGNNRVRAVAMS ATDGLTRGMEVIDTGAPLSVPVGGATLGRIFNVLGEPVDNLGPVDTRTTSPIHRSAPAFTQLDTKLSIFETGIKVVDLLA PYRRGGKIGLFGGAGVGKTVLIMELINNIAKAHGGVSVFGGVGERTREGNDLYMEMKESGVINEQNIAESKVALVYGQMN EPPGARMRVGLTALTMAEYFRDVNEQDVLLFIDNIFRFVQAGSEVSALLGRMPSAVGYQPTLSTEMGSLQERITSTKEGS ITSIQAVYVPADDLTDPAPATTFAHLDATTVLSRGLAAKGIYPAVDPLDSTSTMLQPRIVGEEHYEIAQRVKETLQRYKE LQDIIAILGLDELSEEDRLTVARARKIERFLSQPFFVAEVFTGSPGKYVGLAETIRGFQLILSGELDSLPEQAFYLVGNI DEATAKAMNLEMESKLKK 3.6.3.14 ATP synthase gamma chain, chloroplastic Spinach 0.040119066 1 LEVO MACSLSFSSSVSTFHLPTTTQSTQAPPNNATTLPTTNPIQCANLRELRDRIGSVKNTQKITEAMKLVAAAKVRRAQEAVV NGRPFSETLVEVLYNMNEQLQTEDVDVPLTKIRTVKKVALMVVTGDRGLCGGFNNMLLKKAESRIAELKKLGVDYTIISI GKKGNTYFIRRPEIPVDRYFDGTNLPTAKEAQAIADDVFSLFVSEEVDKVEMLYTKFVSLVKSDPVIHTLLPLSPKGEIC DINGKCVDAAEDELFRLTTKEGKLTVERDMIKTETPAFSPILEFEQDPAQILDALLPLYLNSQILRALQESLASELAARM TAMSNATDNANELKKTLSINYNRARQAKITGEILEIVAGANACV ATP synthase delta chain, chloroplastic Spinach 0.027708582 1 LEVO MAALQNPVALQSRTTTAVAALSTSSTTSTPKPFSLSFSSSTATFNPLRLKILTASKLTAKPRGGALGTRMVDSTASRYAS ALADVADVTGTLEATNSDVEKLIRIFSEEPVYYFFANPVISIDNKRSVLDEIITTSGLQPHTANFINILIDSERINLVKE ILNEFEDVFNKITGTEVAVVTSVVKLENDHLAQIAKGVQKITGAKNVRIKTVIDPSLVAGFTIRYGNEGSKLVDMSVKKQ LEEIAAQLEMDDVTLAV ATP synthase subunit b', chloroplastic Spinach 0.024487596 1 LEVO MANMLVASSSKTLPTTTTTTITPKPKFPLLKTPLLKLSPPQLPPLKHLNLSVLKSAAITATPLTLSFLLPYPSLAEEIEK ASLFDFNLTLPIIMAEFLFLMFALDKIYYTPLGDFMDKRDASIKEQLSGVKDTSSEVKQLEEQANAVMRAARAEISAALN KMKKETQLEVEAKLAEGRKKIEVELQEALGSLEQQKEDTIKSLDSQISALSDDIVKKVLPVS ATP synthase subunit b, chloroplastic Spinach 0.021013904 1 LEVO MKNVTDSFVFLGHWPSAGSFGFNTDILATNLINLSVVLGVLIFFGKGVLSDLLDNRKQRILNTIRNSEELRGKAIEQLEK ARARLKKVEMDADQFRVNGYSEIEREKMNLINSTYKTLEQFENYKNETIQFEQQKAINQVRQRVFQQALQGALGTLNSCL NNELHLRTINANIGMFGAMNEITD ATP synthase subunit a, chloroplastic Spinach 0.02710268 1 LEVO MNVLSYSINPLKGLYAISGVEVGQHFYWQIGGFQIHGQVLITSWVVIAILLGSAAIAVRSPQTIPTGGQNFFEYVLEFIR DVSKTQIGEEYRPWVPFIGTMFLFIFVSNWSGALLPWKIIQLPHGELAAPTNDINTTVALALLTSVAYFYAGLTKKGLGY FGKYIQPTPILLPINILEDFTKPLSLSFRLFGNILADELVVVVLVSLVPLVVPIPVMFLGLFTSGIQALIFATLAAAYIG ESLEGHH ATP synthase epsilon chain, chloroplastic Spinach 0.014715707 1 LEVO MTLNLCVLTPNRSIWNSEVKEIILSTNSGQIGVLPNHAPTATAVDIGILRIRLNDQWLTLALMGGFARIGNNEITILVND AERGSDIDPQEAQQTLEIAEANLRKAEGKRQKIEANLALRRARTRVEASNTISS ATP synthase subunit c, chloroplastic Spinach 0.007977366 14 LEVO MNPLIAAASVIAAGLAVGLASIGPGVGQGTAAGQAVEGIARQPEAEGKIRGTLLLSLAFMEALTIYGLVVALALLFANPF V singleParticle particle 8.0 10.0 C4H12ClNO3Cl Tris-Cl 2.0 MgCl2 Magnesium chloride 500.0 C10H16N5O13P3 ATP 0.4 C24H46O11 beta-DDM C-flat-1.2/1.3 4C COPPER 400 CARBON HOLEY ARRAY GLOW DISCHARGE ETHANE 100 295 FEI VITROBOT MARK IV
Blotting time 6 seconds.
Spinach leaves
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 50.0 2.7 1.5 1.5 4.0 4.0 18000.0 48077.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K2 SUMMIT (4k x 4k) SUPER-RESOLUTION 7676 7420 5.0 2 1637 6.0 43.5
Frames were collected at a rate of 200 ms per frame.
 1 424688 Gctf 1.06
The initial model was ab initially calculated by SPARX VIPER using 2D class averages.
 C1 BACK PROJECTION 6.29 FSC 0.143 CUT-OFF RELION 2.1 25590 COMMON LINE SPARX 2014 COMMON LINE RELION 2.1 6 RELION 2.1 emd_7838.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 288 288 288 0 0 0 288 288 288 339.84 339.84 339.84 90.0 90.0 90.0 X Y Z -0.032392036 0.0801981 0.000661196 0.0040740743 1.18 1.18 1.18 0.0276 AUTHOR primary map 1FX0 A 25-501 1FX0 B 19-485 2W5J A 2-79 OTHER REAL