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    <admin>
        <lastUpdate>2021-02-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-04-30</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2021-02-10</headerReleaseDate>
        <obsoletedDate>2021-02-10</obsoletedDate>
        <title>Spinach chloroplast ATP synthase (reduced)</title>
        <authors>Yang J-H, Williams D, Fromme P, Chiu P-L</authors>
        <fittedPDBEntryIdList>
            <fittedPDBEntryId>6d9v</fittedPDBEntryId>
        </fittedPDBEntryIdList>
        <primaryReference published="false">
            <journalArticle>
                <authors>Yang J-H, Williams D, Fromme P, Chiu P-L</authors>
                <articleTitle>Mechanism of light sensitivity of chloroplast ATP synthase revealed by single-particle cryo-EM</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
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            <spacingRow>288</spacingRow>
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            <cellBeta units="degrees">90.0</cellBeta>
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        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
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            <maximum>0.0801981</maximum>
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        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
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        <contourLevel source="author">0.0276</contourLevel>
        <annotationDetails>primary map</annotationDetails>
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    <sample>
        <numComponents>10</numComponents>
        <name>Chloroplast ATP synthase complex</name>
        <details>Spinach chloroplast ATP synthase from thylakoid membranes</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Chloroplast ATP synthase complex</sciName>
                <molWtTheo units="MDa">0.594</molWtTheo>
                <details>Spinach chloroplast ATP synthase from thylakoid membranes</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinacia oleracea</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <natSource>
                        <organelle>Chloroplast</organelle>
                        <organOrTissue>Leaves</organOrTissue>
                        <cellLocation>Thylakoid membranes</cellLocation>
                    </natSource>
                    <engSource />
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>ATP synthase subunit alpha, chloroplastic</sciName>
                <molWtTheo units="MDa">0.055505199</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>3</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>ATP synthase subunit beta, chloroplastic</sciName>
                <molWtTheo units="MDa">0.053797367</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>3</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>protein</entry>
                <sciName>ATP synthase gamma chain, chloroplastic</sciName>
                <molWtTheo units="MDa">0.040119066</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="5">
                <entry>protein</entry>
                <sciName>ATP synthase delta chain, chloroplastic</sciName>
                <molWtTheo units="MDa">0.027708582</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="6">
                <entry>protein</entry>
                <sciName>ATP synthase subunit b', chloroplastic</sciName>
                <molWtTheo units="MDa">0.024487596</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="7">
                <entry>protein</entry>
                <sciName>ATP synthase subunit b, chloroplastic</sciName>
                <molWtTheo units="MDa">0.021013904</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="8">
                <entry>protein</entry>
                <sciName>ATP synthase subunit a, chloroplastic</sciName>
                <molWtTheo units="MDa">0.02710268</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="9">
                <entry>protein</entry>
                <sciName>ATP synthase epsilon chain, chloroplastic</sciName>
                <molWtTheo units="MDa">0.014715707</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="10">
                <entry>protein</entry>
                <sciName>ATP synthase subunit c, chloroplastic</sciName>
                <molWtTheo units="MDa">0.007977366</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="3562">Spinach</sciSpeciesName>
                    <numCopies>14</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>100</humidity>
            <temperature units="Kelvin">295</temperature>
            <instrument>FEI VITROBOT MARK IV</instrument>
            <details>Blotting time 6 seconds. </details>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">43.5</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <nominalDefocusMin units="nm">1500.0</nominalDefocusMin>
            <nominalDefocusMax units="nm">4000.0</nominalDefocusMax>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>GATAN K2 SUMMIT (4k x 4k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <calibratedMagnification>48077</calibratedMagnification>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
            <nominalMagnification>18000</nominalMagnification>
        </imaging>
        <imageAcquisition>
            <numDigitalImages>1637</numDigitalImages>
            <samplingSize units="microns">5.0</samplingSize>
            <details>Frames were collected at a rate of 200 ms per frame.</details>
        </imageAcquisition>
        <fitting>
            <pdbEntryIdList>
                <pdbEntryId>1FX0</pdbEntryId>
                <pdbEntryId>1FX0</pdbEntryId>
                <pdbEntryId>2W5J</pdbEntryId>
                <pdbChainId>A</pdbChainId>
                <pdbChainId>B</pdbChainId>
                <pdbChainId>A</pdbChainId>
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            <refSpace>REAL</refSpace>
        </fitting>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>8</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <algorithm>BACK PROJECTION</algorithm>
            <software>RELION</software>
            <resolutionByAuthor>6.29</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C1</appliedSymmetry>
            <numProjections>25590</numProjections>
        </singleParticle>
    </processing>
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