<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v3/v3_0_2_2/emdb.xsd" emdb_id="EMD-7787" version="3.0.2.2">
    <admin>
        <current_status>
            <date>2021-02-01</date>
            <code>OBS</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2018-04-13</deposition>
            <header_release>2021-02-10</header_release>
            <obsolete>2021-02-10</obsolete>
            <update>2021-02-01</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Suppressed</funding_body>
                <code>Suppressed</code>
                <country>Suppressed</country>
            </grant_reference>
        </grant_support>
        <title>Suppressed</title>
        <authors_list>
            <author>Suppressed X</author>
            <author>Suppressed X</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Suppressed X</author>
                    <title>Suppressed</title>
                    <journal_abbreviation>Suppressed</journal_abbreviation>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-7787</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
                <details>Suppressed</details>
            </emdb_reference>
        </emdb_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>6d2d</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Suppressed</name>
        <supramolecule_list>
            <virus_supramolecule supramolecule_id="1">
                <name>Suppressed</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <details>Suppressed</details>
                <sci_species_name ncbi="32644">Suppressed</sci_species_name>
                <sci_species_strain>Suppressed</sci_species_strain>
                <natural_host database="NCBI">
                    <organism ncbi="32644">Suppressed</organism>
                </natural_host>
                <molecular_weight>
                    <theoretical units="MDa">1</theoretical>
                </molecular_weight>
                <virus_shell shell_id="1">
                    <name>coat protein</name>
                    <triangulation>7</triangulation>
                </virus_shell>
                <virus_type>VIRION</virus_type>
                <virus_isolate>STRAIN</virus_isolate>
                <virus_enveloped>false</virus_enveloped>
                <virus_empty>false</virus_empty>
            </virus_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Suppressed</name>
                <natural_source database="NCBI">
                    <organism ncbi="32644">Suppressed</organism>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">1e-06</theoretical>
                </molecular_weight>
                <number_of_copies>3</number_of_copies>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>X</string>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">1</concentration>
                    <buffer>
                        <ph>7.6</ph>
                        <component>
                            <concentration units="mM">10.0</concentration>
                            <formula>MgCl2</formula>
                            <name>magnesium chloride</name>
                        </component>
                        <component>
                            <concentration units="mM">10.0</concentration>
                            <formula>C4H11NO3</formula>
                            <name>Tris</name>
                        </component>
                    </buffer>
                    <grid>
                        <model>Quantifoil R2/2</model>
                        <material>COPPER</material>
                        <mesh>200</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                            <film_thickness units="nm">100.0</film_thickness>
                        </support_film>
                        <pretreatment>
                            <type>PLASMA CLEANING</type>
                        </pretreatment>
                        <details>standard conditions with a Fischione model 1020 plasma cleaner</details>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>HOMEMADE PLUNGER</instrument>
                    </vitrification>
                    <details>concentration is 1x10^12 phage/mL</details>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">0.35</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.49</nominal_defocus_max>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>DIRECT ELECTRON DE-20 (5k x 3k)</film_or_detector_model>
                            <detector_mode>INTEGRATING</detector_mode>
                            <digitization_details>
                                <dimensions>
                                    <width units="pixel">5000</width>
                                    <height units="pixel">4000</height>
                                </dimensions>
                                <sampling_interval units="µm">6.4</sampling_interval>
                                <frames_per_image>0-53</frames_per_image>
                            </digitization_details>
                            <number_grids_imaged>1</number_grids_imaged>
                            <number_real_images>494</number_real_images>
                            <average_exposure_time units="s">2.0</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">27.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <details>images were motion corrected and also particles were corrected for damage compensation</details>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>Auto3DEM</name>
                            <version>v4.01.07</version>
                        </software>
                    </software_list>
                    <details>estimated manually</details>
                </ctf_correction>
                <startup_model type_of_model="OTHER">
                    <details>random model computation</details>
                </startup_model>
                <final_reconstruction>
                    <applied_symmetry>
                        <point_group>I</point_group>
                    </applied_symmetry>
                    <algorithm>BACK PROJECTION</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">4.2</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>Auto3DEM</name>
                            <version>v4.01.07</version>
                        </software>
                    </software_list>
                    <number_images_used>7879</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing/>
                    <software_list>
                        <software>
                            <name>Auto3DEM</name>
                            <version>v4.01.07</version>
                        </software>
                    </software_list>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>PROJECTION MATCHING</type>
                    <projection_matching_processing/>
                    <software_list>
                        <software>
                            <name>Auto3DEM</name>
                            <version>v4.01.07</version>
                        </software>
                    </software_list>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="1377889">
        <file>emd_7787.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>701</col>
            <row>701</row>
            <sec>701</sec>
        </dimensions>
        <origin>
            <col>-101</col>
            <row>-101</row>
            <sec>-101</sec>
        </origin>
        <spacing>
            <x>701</x>
            <y>701</y>
            <z>701</z>
        </spacing>
        <cell>
            <a units="Å">883.26</a>
            <b units="Å">883.26</b>
            <c units="Å">883.26</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-406.6061</minimum>
            <maximum>838.45526</maximum>
            <average>7.9814777</average>
            <std>135.14032</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.26</x>
            <y units="Å">1.26</y>
            <z units="Å">1.26</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>311.0</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-7787::::</label>
        <annotation_details>Suppressed</annotation_details>
    </map>
</emd>
