2016-03-09 OBS 2015-11-03 RCSB PDBj 2015-12-09 2016-03-09
Obsoleted as per author's request
Electron microscopy structure of monomeric ATM kinase Lau WCY, Li Y, Liu Z, Gao Y, Zhang Q, Huen MSY DNA damage, ATM kinase, Electron microscopy 3jc0 Lau WCY, Li Y, Liu Z, Gao Y, Zhang Q, Huen MSY Structural insights into the regulatory mechanism of ATM kinase activation To Be Published emd_6502.map.gz Image stored as Reals 100 100 100 -50 -50 -50 49 49 49 100 100 100 461.0 461.0 461.0 90.0 90.0 90.0 X Y Z -0.08145520 1.07092738 0.00547454 0.05157611 1
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4.61 4.61 4.61 0.45 Reconstruction of monomeric ATM kinase 1 Monomeric ATM kinase 1 0.35
The sample was monodisperse
protein Monomeric ATM kinase 0.35 Homo sapiens true Monomer Human 1 pcDNA-FLAG-His-ATM wt Homo sapiens 293T NONE NONE LAB6 25 BRIGHT FIELD 1000 4000 FLOOD BEAM GATAN ULTRASCAN 4000 (4k x 4k) 2.0 296 JEOL 2010 01-JUL-2015 GATAN LIQUID NITROGEN 200 50000 251 cross correlation coefficient
Residues 2021-2118 are removed
Chimera rigid body REAL 4JSV A 2% w/v uranyl acetate particle 0.02 continuous carbon coated copper grids
25 mM Tris, 100 mM NaCl, 1 mM TCEP, 10% glycerol
8.0 singleParticle projection matching PRIME, EMAN2 class average 28 FSC 0.143, gold-standard C1 6546