HPUB OBS PDBj RCSB PDBj 2015-11-03 2015-12-09 2016-03-09 2016-03-09 Electron microscopy structure of monomeric ATM kinase Lau WCY Li Y Liu Z Gao Y Zhang Q Huen MSY DNA damage, ATM kinase, Electron microscopy Lau WCY Li Y Liu Z Gao Y Zhang Q Huen MSY Structural insights into the regulatory mechanism of ATM kinase activation To Be Published 3jc0 FULLOVERLAP Monomeric ATM kinase Monomeric ATM kinase
The sample was monodisperse
1 1 0.35 Monomeric ATM kinase Homo sapiens Human 0.35 1 Monomer true Homo sapiens 293T pcDNA-FLAG-His-ATM wt singleParticle particle 0.02 8.0
25 mM Tris, 100 mM NaCl, 1 mM TCEP, 10% glycerol
 NEGATIVE
2% w/v uranyl acetate
continuous carbon coated copper grids
 NONE OTHER JEOL 2010 FLOOD BEAM BRIGHT FIELD LAB6 200 2.0 1.0 4.0 50000.0 GATAN LIQUID NITROGEN 296 2015-07-01 GATAN ULTRASCAN 4000 (4k x 4k) 251 25
class average
 OTHER 28.0 OTHER PRIME, EMAN2 6546 emd_6502.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 -50 -50 -50 100 100 100 461.0 461.0 461.0 90.0 90.0 90.0 X Y Z -0.0814552 1.07092738 0.00547454 0.05157611 4.61 4.61 4.61 0.45 AUTHOR Reconstruction of monomeric ATM kinase
::::EMDATABANK.org::::EMD-6502::::
 4JSV A RIGID BODY FIT Chimera
Residues 2021-2118 are removed
cross correlation coefficient REAL