In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus

HPUB OBS RCSB RCSB RCSB 2015-07-29 2015-08-19 2015-10-28 2015-10-28 In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus Zhang X Ding K Yu XK Chang W Sun JC Zhou ZH cryo-EM, dsRNA genome organization, viral polymerase Zhang X Ding K Yu X Chang W Sun J Zhou ZH In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus NATURE 2015 doi:10.1038/nature15767 CPV VP4 + polymerase complex (TEC) CPV VP4 + polymerase complex (TEC) 2 VP4 Bombyx mori cypovirus 1 Cytoplasmic polyhedrosis virus false RNA polymerase Bombyx mori cypovirus 1 Cytoplasmic polyhedrosis virus false singleParticle particle 8.0

70 mM Tris-Cl, 10 mM MgCl2, 100 mM NaCl, 2 mM GTP

200 mesh Quantifoil holey carbon film

ETHANE FEI VITROBOT MARK II FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.6 4.5 49000.0 49500.0 FEI TITAN KRIOS AUTOGRID HOLDER 80 0 2013-07-26 GATAN K2 (4k x 4k) 5 4385 25

Each particle

3.3 OTHER Frealign 68526 1 emd_6401.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 164 131 142 -111 46 107 164 131 142 132.31 165.64 143.42 90.0 90.0 90.0 Y X Z -8.85126591 15.05303574 0.0 1.0 1.01 1.01 1.01 1.5 AUTHOR Averaged CPV VP4 + polymerase

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emd_6401.jpg