HPUB OBS PDBj RCSB PDBj 2015-06-24 2015-11-25 2016-06-15 2016-06-15 The cryoEM map of EV71 empty particle in complex with the Fab fragment of antibody D5 Fan C Ye XH Ku ZQ Zuo T Kong LL Zhang C Shi JP Liu QW Chen T Zhang YY Jiang W Zhang LQ Huang Z Cong Y Enterovirus 71(EV71), virus-antibody complex, bivalent binding, high resolution cryo-EM Ye XH Fan C Ku ZQ Zuo T Kong LL Zhang C Shi JP Liu QW Chen T Zhang YY Jiang W Zhang LQ Huang Z Cong Y Structural basis for recognition of human enterovirus 71 by a bivalent broadly neutralizing monoclonal antibody To Be Published EV71 empty particle in complex with the Fab fragment of antibody D5 EV71 empty particle in complex with the Fab fragment of antibody D5 One fab fragment of antibody D5 bind to one protomer of EV71 2 8 Human enterovirus 71
EV71 empty particle in complex with the Fab fragment of antibody D5
Human enterovirus 71 G082 Homo sapiens VERTEBRATES 5 300 3 VIRION STRAIN false true antibody D5 Homo sapiens Human false singleParticle particle 0.8 7.4
0.15 M phosphate buffered saline(PBS) buffer
 NEGATIVE
Grids were plunge-frozen into liquid ethane using a FEI Mark IV virtobot, after 2s blotting to remove extra sample.
200 mesh R1.2x1.3 Quantifoil Cu grid, glow discharged in air.
 ETHANE 100 120 FEI VITROBOT MARK IV
Grids were plunge-frozen into liquid ethane using a FEI Mark IV virtobot, after 2s blotting to remove extra sample.
Blot for 2 seconds before plunging FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0.005 2.0 3.5 37000.0 FEI TITAN KRIOS AUTOGRID HOLDER 90 92 91 Objective lens astigmatism was corrected at 75000 magnification 0 2014-10-15 FEI FALCON II (4k x 4k) 320 16
The particles were boxed using e2boxer.py. CTF fitting was automatically performed using fitctf2.py in jspr, then visually validated and adjusted using EMAN1.9 ctfit program. The gold standard 3D reconstruction procedure was followed using jspr package, with the datasets split into two halves in the beginning.
Each particle
 OTHER 6.0 OTHER jspr, EMAN, EMAN2 1303 emd_6364.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 -160 -160 -160 320 320 320 572.8 572.8 572.8 90.0 90.0 90.0 X Y Z -1.22612703 1.93103516 0.02268821 0.14356451 1.79 1.79 1.79 0.5 AUTHOR The cryoEM map of EV71 empty particle in complex with the Fab fragment of antibody D5
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 3VBU C RIGID BODY FIT Chimera REAL
emd_6364.jpg