2016-06-15 OBS 2015-06-23 RCSB PDBj 2015-11-25 2016-06-15
author deposited better maps
The cryoEM map of EV71 VLP in complex with intact antibody D5 Fan C, Ye XH, Ku ZQ, Zuo T, Kong LL, Zhang C, Shi JP, Liu QW, Chen T, Zhang YY, Jiang W, Zhang LQ, Huang Z, Cong Y Enterovirus 71(EV71), virus-antibody complex, bivalent binding, high resolution cryo-EM Ye XH, Fan C, Ku ZQ, Zuo T, Kong LL, Zhang C, Shi JP, Liu QW, Chen T, Zhang YY, Jiang W, Zhang LQ, Huang Z, Cong Y Structural basis for recognition of human enterovirus 71 by a bivalent broadly neutralizing monoclonal antibody To Be Published emd_6363.map.gz Image stored as Reals 320 320 320 -160 -160 -160 159 159 159 320 320 320 572.8 572.8 572.8 90.0 90.0 90.0 X Y Z -0.90984285 2.88650131 0.04957140 0.20625038 1
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1.79 1.79 1.79 0.8 The cryoEM map of EV71 VLP in complex with intact antibody D5
emd_6363.tif
2 EV71 VLP in complex with intact antibody D5 One fab fragment of antibody D5 bind to one protomer of EV71 8 virus Human enterovirus 71 5
EV71 VLP in complex with intact antibody D5
Human enterovirus 71 G082 false true VIRUS-LIKE PARTICLE STRAIN VERTEBRATES Homo sapiens Komagataella pastoris pPink-HC PichiaPink Strain 1 3 300 protein antibody D5 false Homo sapiens Human Blot for 2 seconds before plunging ETHANE
Grids were plunge-frozen into liquid ethane using a FEI Mark IV virtobot, after 2s blotting to remove extra sample.
100 FEI VITROBOT MARK IV 120 Objective lens astigmatism was corrected at 75000 magnification FIELD EMISSION GUN 16 BRIGHT FIELD 2000 90 3500 FLOOD BEAM 92 FEI FALCON II (4k x 4k) 0.005 0 0 91 FEI TITAN KRIOS 01-OCT-2014 0 FEI TITAN KRIOS AUTOGRID HOLDER 300 37000 192
Every image is the average of seven frames recorded by the direct electron detector
 Chimera rigid body REAL 3VBU A B C Grids were plunge-frozen into liquid ethane using a FEI Mark IV virtobot, after 2s blotting to remove extra sample. particle 1.4 200 mesh R1.2x1.3 Quantifoil Cu grid, glow discharged in air.
0.15 M phosphate buffered saline(PBS) buffer
7.6 singleParticle Cross-common lines jspr, EMAN, EMAN2 Each particle 5.5 FSC 0.143, gold-standard I 2241
The particles were boxed using e2boxer.py. CTF fitting was automatically performed using fitctf2.py in jspr, then visually validated and adjusted using EMAN1.9 ctfit program. The gold standard 3D reconstruction procedure was followed using jspr package, with the datasets split into two halves in the beginning.