2016-07-06 OBS 2015-06-23 RCSB RCSB 2015-07-01 2016-06-29 2016-07-06

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An JY, Sharif H, Barrera FN, Karabadzhak AG, Song JJ, Jung TY, Hebert H, Kang GB, Park KJ, Lee JG, Sakkiah S, Lee KW, Jeon H, Lee S, Engelman DM, Wang J, Eom SH ATP-dependent proteolysis, Non-native membrane proteins, Open closed gating, TM modeling, Crystallography and electron microscopy An JY, Sharif H, Barrera FN, Karabadzhak AG, Song JJ, Jung TY, Hebert H, Kang GB, Park KJ, Lee JG, Sakkiah S, Lee KW, Jeon H, Lee S, Engelman DM, Wang J, Eom SH Crystallographic and EM structures of FtsH and its domains suggest a reverse translocon mechanism for membrane protein extraction To Be Published

emd_6362.png

emd_6362_fsc.xml 1 Full length FtsH from Thermotoga maritima homohexamer 0.65 size exclusion chromatography 0.65 protein FtsH protease 0.65 0.65 Thermotoga maritima true homohexamer Q9WZ49 pET28a Escherichia coli NONE NONE Objective lens astigmatism was corrected at 50000 times magnification. FIELD EMISSION GUN BRIGHT FIELD 600 1700 FLOOD BEAM TVIPS TEMCAM-F415 (4k x 4k) 2.0 72165 JEOL 2100F 09-DEC-2014 JEOL 200 50000 50 Grids with absorbed protein were pre-washed 3 times with DW and stained with 2% uranyl acetate for one minute. particle 400 mesh copper grid with thin carbon

50 mM HEPES, 100 mM NaCl, 0.1% glutaldehyde, 10% sucrose

7.5 singleParticle EMAN2 Each particle 21 FSC 0.5, gold-standard 20 C6 2558

All data processing and refinement was done using the EMAN2 program.