Negative-stain electron microscopy of the full-length Thermotoga maritima FtsH

REL OBS RCSB RCSB RCSB 2015-06-23 2015-07-01 2016-06-29 2016-07-06 2016-07-06 Negative-stain electron microscopy of the full-length Thermotoga maritima FtsH An JY Sharif H Barrera FN Karabadzhak AG Song JJ Jung TY Hebert H Kang GB Park KJ Lee JG Sakkiah S Lee KW Jeon H Lee S Engelman DM Wang J Eom SH ATP-dependent proteolysis, Non-native membrane proteins, Open closed gating, TM modeling, Crystallography and electron microscopy An JY Sharif H Barrera FN Karabadzhak AG Song JJ Jung TY Hebert H Kang GB Park KJ Lee JG Sakkiah S Lee KW Jeon H Lee S Engelman DM Wang J Eom SH Crystallographic and EM structures of FtsH and its domains suggest a reverse translocon mechanism for membrane protein extraction To Be Published Full length FtsH from Thermotoga maritima Full length FtsH from Thermotoga maritima homohexamer 1 0.65 0.65 size exclusion chromatography FtsH protease Thermotoga maritima 0.65 0.65 homohexamer true Escherichia coli pET28a Q9WZ49 singleParticle particle 7.5

50 mM HEPES, 100 mM NaCl, 0.1% glutaldehyde, 10% sucrose

NEGATIVE

Grids with absorbed protein were pre-washed 3 times with DW and stained with 2% uranyl acetate for one minute.

400 mesh copper grid with thin carbon

NONE OTHER JEOL 2100F FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 0.6 1.7 50000.0 72165.0 JEOL Objective lens astigmatism was corrected at 50000 times magnification. 2014-12-09 TVIPS TEMCAM-F415 (4k x 4k) 50

All data processing and refinement was done using the EMAN2 program.

Each particle

21.0 OTHER EMAN2 2558 20 emd_6362.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 150 150 150 -75 -75 -75 150 150 150 312.0 312.0 312.0 90.0 90.0 90.0 X Y Z -2.77754521 7.27051163 0.02960115 0.34238788 2.08 2.08 2.08 0.9 AUTHOR Reconstruction of full length FtsH from Thermotoga maritima

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emd_6362.png emd_6362_fsc.xml