2016-03-23 OBS 2015-03-13 RCSB PDBj 2015-05-06 2016-03-23 2016-03-23
authors are working on a higher-resolution map and not planning to publish this low-resolution structure
The cryo-EM structure of Meiothermus taiwanensis Lon protease without ATP and Mg2+ Su SC, Chang YC, Chang CI ATP-dependent protease, sequestered degradation chamber, AAA+ domain, protease domain Su SC, Chang YC, Chang CI Structural modulators control the assembly and activity of the Lon AAA+ protease To Be Published emd_6302.map.gz Image stored as Reals 120 120 120 -60 -60 -60 59 59 59 120 120 120 198.0 198.0 198.0 90.0 90.0 90.0 X Y Z -65.58416748 240.97012329 10.53799057 35.73754883 1
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1.65 1.65 1.65 170 Reconstruction of Meiothermus taiwanensis LonA protease
emd_6302.tiff
1 Meiothermus taiwanensis LonA protease One homotetramer of MtaLonA in apo form 0.54 Sedimentation 0.54 protein Meiothermus taiwanensis LonA protease MtaLonA 0.54 0.54 Meiothermus taiwanensis true Tetramer Meiothermus taiwanensis PET21a Escherichia coli 1. wait for 10 seconds before blot 2. Blot for 3 seconds before plunging ETHANE 100 FEI VITROBOT MARK IV Objective lens astigmatism was corrected at 100,000 times magnification FIELD EMISSION GUN 20 BRIGHT FIELD 1000 5000 FLOOD BEAM GATAN ULTRASCAN 4000 (4k x 4k) 2 80000 FEI TECNAI F20 09-OCT-1995 GATAN LIQUID NITROGEN 200 62000 122 Grids with adsorbed protein floated on 2% w/v uranyl acetate for 60 seconds. particle 0.12 200 mesh gold grid with thin carbon support, glow discharged in amylamine atmosphere
20mM Tris-HCl, 100mM NaCl, 1mM DTT
8.0 singleParticle EMAN2 Each particle 15.1 FSC 0.5, semi-independent 80 C4 14790
The particle images were interactively selected using EMAN Boxer program