REL OBS PDBj RCSB PDBj 2015-03-13 2015-05-06 2016-03-23 2016-03-23 2016-03-23 The cryo-EM structure of Meiothermus taiwanensis Lon protease without ATP and Mg2+ Su SC Chang YC Chang CI ATP-dependent protease, sequestered degradation chamber, AAA+ domain, protease domain Su SC Chang YC Chang CI Structural modulators control the assembly and activity of the Lon AAA+ protease To Be Published Meiothermus taiwanensis LonA protease Meiothermus taiwanensis LonA protease One homotetramer of MtaLonA in apo form 1 0.54 0.54 Sedimentation Meiothermus taiwanensis LonA protease Meiothermus taiwanensis Meiothermus taiwanensis 0.54 0.54 Tetramer true Escherichia coli PET21a singleParticle particle 0.12 8.0
20mM Tris-HCl, 100mM NaCl, 1mM DTT
 NEGATIVE
Grids with adsorbed protein floated on 2% w/v uranyl acetate for 60 seconds.
200 mesh gold grid with thin carbon support, glow discharged in amylamine atmosphere
 ETHANE 100 FEI VITROBOT MARK IV 1. wait for 10 seconds before blot 2. Blot for 3 seconds before plunging FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2 1.0 5.0 62000.0 80000.0 GATAN LIQUID NITROGEN Objective lens astigmatism was corrected at 100,000 times magnification 1995-10-09 GATAN ULTRASCAN 4000 (4k x 4k) 122 20
The particle images were interactively selected using EMAN Boxer program
Each particle
 15.1 OTHER EMAN2 14790 80 emd_6302.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 120 120 120 -60 -60 -60 120 120 120 198.0 198.0 198.0 90.0 90.0 90.0 X Y Z -65.58416748 240.97012329 10.53799057 35.73754883 1.65 1.65 1.65 170.0 AUTHOR Reconstruction of Meiothermus taiwanensis LonA protease
::::EMDATABANK.org::::EMD-6302::::
emd_6302.tiff