HPUB OBS RCSB RCSB RCSB 2015-01-24 2015-03-18 2016-07-20 2016-07-20 Cryo-EM structure of Dengue virus serotype 1 complexed with Fab fragments of human antibody D11C Dip PV Fibriansah G Ng TS Wang J Kukkaro P Kostyuchenko VA Robinson JE Isern S Michael SF Anand G Schieffelin JS Lok SM antibody, Dengue virus, fusion loop Dip PV Fibriansah G Ng TS Wang J Kukkaro P Kostyuchenko VA Robinson JE Isern S Michael SF Anand G Schieffelin JS Lok SM Antibody-Dengue virus complex shows neutralization mechanism by the pre-dominant human antibodies To Be Published 3j9h FULLOVERLAP Dengue virus serotype 1 with variable domain of Fab fragments of human monoclonal antibody D11C Dengue virus serotype 1 with variable domain of Fab fragments of human monoclonal antibody D11C 60 Fab molecules bind to one Dengue virion 2 Dengue virus 1 Dengue virus 1 PVP159 Aedes aegypti INVERTEBRATES Aedes albopictus C6/36 cells VIRION STRAIN true false antibody D11C Fab, variable region Homo sapiens human 60 false singleParticle particle 8.0
12 mM Tris-HCl, pH 8.0, 120 mM NaCl, 1 mM EDTA
 ETHANE 100 100 FEI VITROBOT MARK IV Blot with filter paper for 2 seconds before plunging. FEI TITAN KRIOS SPOT SCAN BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.0 4.0 47000.0 FEI TITAN KRIOS AUTOGRID HOLDER 100 FEI 2013-08-10 FEI FALCON I (4k x 4k) 18.1
The particles were selected manually.
Each particle
 OTHER 21.0 OTHER EMAN2, RELION, MPSA 8537 100 emd_6257.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 512 512 512 -256 -256 -256 512 512 512 875.52 875.52 875.52 90.0 90.0 90.0 X Y Z -10.19386959 16.27508354 -0.13167311 1.00621843 1.71 1.71 1.71 1.5 AUTHOR Cryo-EM structure of Dengue virus serotype 1 complexed with variable region of Fab fragments of human antibody D11C
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 1OAN FLEXIBLE FIT O, Chimera, MDFF REAL