2015-06-10 OBS 2014-05-29 RCSB RCSB 2014-07-30 2015-06-10 2015-06-10
One-year hold expired; author will deposit new maps.
EMD-5979, EMD-5980 3DEM structure of Rrp5 Khoshnevis S, Askenasy I, Johnson MC, Young-Erdos CL, Karbstein K, Stroupe ME Rrp5, RNA binding protein, ribosome assembly factor, 40S ribosome, 60S ribosome, Rok1, DEAD-box protein Khoshnevis S, Askenasy I, Johnson MC, Young-Erdos CL, Karbstein K, Stroupe ME ATP Hydrolysis by the DEAD-box protein Rok1 Releases the Assembly Factor Rrp5 from pre-40S Ribosomes. To Be Published emd_5978.map.gz Image stored as Reals 128 128 128 -23 -23 -23 104 104 104 128 128 128 345.6 345.6 345.6 90.0 90.0 90.0 X Y Z -6.87094307 18.12568092 0E-8 1.00000000 1
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2.7 2.7 2.7 5.81 Reconstruction of Rrp5:RNA 2 Saccharomyces cerevisiae Rrp5 bound to H45-3ITS1 RNA 1 0.35
At a 5 sigma contour, the mass is about 0.25 MDa, which corresponds to about 0.2 MDa from Rrp5 and 0.05 MDa from RNA.
protein Ribosomal RNA Processing 5 Rrp5, U3 small nucleolar RNA-associated protein RRP5 0.35 1 monomer By 4741 Saccharomyces cerevisiae Yeast true GO:0006396 GO:0003723 GO:0005515 GO:0005622 Q05022 IPR012340 IPR003029 IPR011990 IPR003107 nucleolus Escherichia coli Rosetta2 (DE3) pGEX-6-P3 nucleic-acid pre-rRNA H45-3ITS1 RNA 0.12
in vitro transcribed
RNA SINGLE STRANDED By 4741 Saccharomyces cerevisiae false yeast NONE NONE FIELD EMISSION GUN 20 BRIGHT FIELD 1000 3000 FLOOD BEAM GATAN ULTRASCAN 4000 (4k x 4k) 65555 FEI TITAN KRIOS 23-MAR-2013 FEI TITAN KRIOS AUTOGRID HOLDER 120 59000 1200 uranyl formate on floated continuous carbon particle 400 mesh copper grid, glow-discharged in Gatan Solarus apparatus
300 mM NaCl, 10 mM MgCl2, 30 mM MES
6.7 singleParticle RCT followed by projection matching EMAN
Final maps were calculated from two averaged datasets.
30 FSC 0.5, gold-standard 50 C1 8500
Particles were initially manually picked to determine de novo class averages and then automatically picked. Initial model was determined with random conical tilt.