REL OBS RCSB RCSB RCSB 2014-05-29 2014-07-30 2015-06-10 2015-06-10 2015-06-10 Khoshnevis S Askenasy I Johnson MC Young-Erdos CL Karbstein K Stroupe ME Rrp5, RNA binding protein, ribosome assembly factor, 40S ribosome, 60S ribosome, Rok1, DEAD-box protein Khoshnevis S Askenasy I Johnson MC Young-Erdos CL Karbstein K Stroupe ME To Be Published EMD-5979 FULLOVERLAP EMD-5980 FULLOVERLAP Saccharomyces cerevisiae Rrp5 bound to H45-3ITS1 RNA Saccharomyces cerevisiae Rrp5 bound to H45-3ITS1 RNA

At a 5 sigma contour, the mass is about 0.25 MDa, which corresponds to about 0.2 MDa from Rrp5 and 0.05 MDa from RNA.

1 2 0.35 Ribosomal RNA Processing 5 Saccharomyces cerevisiae By 4741 Yeast nucleolus 0.35 1 monomer true Escherichia coli Rosetta2 (DE3) pGEX-6-P3 Q05022 GO:0006396 GO:0003723 GO:0005515 GO:0005622 IPR012340 IPR003029 IPR011990 IPR003107 pre-rRNA Saccharomyces cerevisiae By 4741 yeast 0.12

in vitro transcribed

OTHER SINGLE STRANDED false singleParticle particle 6.7

300 mM NaCl, 10 mM MgCl2, 30 mM MES

NEGATIVE

uranyl formate on floated continuous carbon

400 mesh copper grid, glow-discharged in Gatan Solarus apparatus

NONE OTHER FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 120 1.0 3.0 59000.0 65555.0 FEI TITAN KRIOS AUTOGRID HOLDER 2013-03-23 GATAN ULTRASCAN 4000 (4k x 4k) 1200 20

Particles were initially manually picked to determine de novo class averages and then automatically picked. Initial model was determined with random conical tilt.

OTHER 30.0 OTHER EMAN

Final maps were calculated from two averaged datasets.

8500 50