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        <depositionDate>2014-05-20</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>PDBj</processingSite>
        <headerReleaseDate>2015-01-21</headerReleaseDate>
        <obsoletedDate>2016-01-13</obsoletedDate>
        <details>Authors request for the map to be withdrawn. However the header of the map has been already released.</details>
        <title>Structure of taste receptor T1r2a/T1r3 heterodimer ligand binding domains, L-glutamine-bound form</title>
        <authors>Nango E, Maki-Yonekura S, Ashikawa Y, Nuemket N, Kusakabe Y, Shimizu M, Krayukhina E, Uchiyama S, Akiyama S, Yonekura K, Hikima T, Yamamoto M, Yamashita A</authors>
        <keywords>taste receptor, GPCR</keywords>
        <primaryReference published="false">
            <journalArticle>
                <authors>Nango E, Maki-Yonekura S, Ashikawa Y, Nuemket N, Kusakabe Y, Shimizu M, Krayukhina E, Uchiyama S, Akiyama S, Yonekura K, Hikima T, Yamamoto M, Yamashita A</authors>
                <articleTitle>Structural organization and conformational change of taste receptor T1r heterodimer extracellular domains</articleTitle>
                <journal>To Be Published</journal>
                <volume/>
                <firstPage/>
                <lastPage/>
                <year/>
            </journalArticle>
        </primaryReference>
    </deposition>
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        <annotationDetails>Reconstruction of taste receptor T1r2a/T1r3 heterodimer ligand binding domains, L-glutamine-bound form</annotationDetails>
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    <supplement>
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        <numComponents>2</numComponents>
        <name>Ligand binding domains of taste receptor T1r2a/T1r3 heterodimer from medaka fish</name>
        <compDegree>heterodimer</compDegree>
        <molWtMethod>Mass spectroscopy, SDS-PAGE</molWtMethod>
        <molWtExp units="MDa">0.127</molWtExp>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>taste receptor, type 1, member 2a</sciName>
                <synName>T1r2a, Tas1r2a</synName>
                <protein>
                    <numCopies>1</numCopies>
                    <oligomericDetails>protomer in a heterodimer</oligomericDetails>
                    <sciSpeciesName ncbiTaxId="8090">Oryzias latipes</sciSpeciesName>
                    <synSpeciesName>medaka fish</synSpeciesName>
                    <recombinantExpFlag>true</recombinantExpFlag>
                    <externalReferences>
                        <refGo>GO:0007165</refGo>
                        <refGo>GO:0007186</refGo>
                        <refGo>GO:0004871</refGo>
                        <refGo>GO:0004930</refGo>
                        <refGo>GO:0005886</refGo>
                        <refGo>GO:0016020</refGo>
                        <refGo>GO:0016021</refGo>
                        <refUniProt>Q2MHK5</refUniProt>
                        <refInterpro>IPR000337</refInterpro>
                        <refInterpro>IPR011500</refInterpro>
                        <refInterpro>IPR017978</refInterpro>
                    </externalReferences>
                    <natSource/>
                    <engSource>
                        <expSystem ncbiTaxId="7227">Drosophila melanogaster</expSystem>
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                    </engSource>
                </protein>
            </sampleComponent>
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                <sciName>taste receptor, type 1, member 3</sciName>
                <synName>T1r3, Tas1r3</synName>
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                    <sciSpeciesName ncbiTaxId="8090">Oryzias latipes</sciSpeciesName>
                    <synSpeciesName>medaka fish</synSpeciesName>
                    <externalReferences>
                        <refInterpro>IPR000337</refInterpro>
                        <refInterpro>IPR011500</refInterpro>
                        <refInterpro>IPR017978</refInterpro>
                        <refUniProt>Q2MHK2</refUniProt>
                        <refGo>GO:0007165</refGo>
                        <refGo>GO:0007186</refGo>
                        <refGo>GO:0004871</refGo>
                    </externalReferences>
                    <natSource/>
                    <engSource>
                        <vector>pAc5.1/V5-His</vector>
                        <expSystemCell>S2</expSystemCell>
                        <expSystem ncbiTaxId="7227">Drosophila melanogaster</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
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            <instrument>NONE</instrument>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <specimenHolder>high tilt holder EM-21311HTR</specimenHolder>
            <detector>OTHER</detector>
            <nominalCs units="mm">3.3</nominalCs>
            <microscope>JEOL 2100</microscope>
            <date>23-APR-2013</date>
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            <samplingSize units="microns">30</samplingSize>
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        <specimenPreparation>
            <staining>Purified protein solution was applied onto a carbon-coated grid and negatively stained with 2% uranyl acetate.</staining>
            <specimenState>particle</specimenState>
            <specimenSupportDetails>400 mesh Cu grid</specimenSupportDetails>
            <buffer>
                <details>20mM Tris, 0.3M NaCl, 2mM CaCl2, 5mM glutamine</details>
                <ph>8.0</ph>
            </buffer>
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            <algorithm>projection matching refinement</algorithm>
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