HPUB OBS PDBj RCSB PDBj 2014-05-20 2015-01-21 2016-01-13 2016-01-13 Structure of taste receptor T1r2a/T1r3 heterodimer ligand binding domains, ligand-free form Nango E Maki-Yonekura S Ashikawa Y Nuemket N Kusakabe Y Shimizu M Krayukhina E Uchiyama S Akiyama S Yonekura K Hikima T Yamamoto M Yamashita A taste receptor, GPCR Nango E Maki-Yonekura S Ashikawa Y Nuemket N Kusakabe Y Shimizu M Krayukhina E Uchiyama S Akiyama S Yonekura K Hikima T Yamamoto M Yamashita A Structural organization and conformational change of taste receptor T1r heterodimer extracellular domains To Be Published Ligand binding domains of taste receptor T1r2a/T1r3 heterodimer from medaka fish Ligand binding domains of taste receptor T1r2a/T1r3 heterodimer from medaka fish heterodimer 2 0.127 Mass spectroscopy, SDS-PAGE taste receptor, type 1, member 2a Oryzias latipes medaka fish 1 protomer in a heterodimer true Drosophila melanogaster S2 pAc5.1/V5-His Q2MHK5 GO:0007165 GO:0007186 GO:0004871 GO:0004930 GO:0005886 GO:0016020 GO:0016021 IPR000337 IPR011500 IPR017978 taste receptor, type 1, member 3 Oryzias latipes medaka fish 1 protomer in a heterodimer true Drosophila melanogaster S2 pAc5.1/V5-His Q2MHK2 GO:0007165 GO:0007186 GO:0004871 GO:0004930 GO:0005886 GO:0016020 GO:0016021 IPR000337 IPR011500 IPR017978 singleParticle particle 8.0
20 mM Tris, 0.3 M NaCl, 2 mM CaCl2
 NEGATIVE
Purified protein solution was applied onto a carbon-coated grid and negatively stained with 2% uranyl acetate.
400 mesh Cu grid
 ETHANE OTHER JEOL 2100 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 3.3 50000.0 JEOL 2013-02-26 OTHER 30 150 high tilt holder EM-21311HTR OTHER 24.0 OTHER EMAN 8444 emd_5974.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 -7 -7 -7 64 64 64 295.36 295.36 295.36 90.0 90.0 90.0 X Y Z -4.83898163 22.95459557 0.0 1.04833198 4.615 4.615 4.615 6.965 AUTHOR Reconstruction of taste receptor T1r2a/T1r3 heterodimer ligand binding domains, ligand-free form
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