2014-10-22 OBS 2014-02-06 RCSB RCSB 2014-03-19 2014-10-22 EMD-6101
The same entry was deposited twice.
CryoEM of Actin-Coronin in ADP-BeFx state Ge P, Durer ZAO, Kudryashov D, Zhou ZH, Reisler E Actin, coronin, ADP-BeFx, ADP-Pi, cytoskeleton Ge P, Durer ZAO, Kudryashov D, Zhou ZH, Reisler E CryoEM reveals different coronin binding modes for ADP- and ADP-BeFx- actin filaments To Be Published emd_5894.map.gz Image stored as Reals 320 320 320 -160 -160 -160 159 159 159 320 320 320 459.84003 459.84003 459.84003 90.0 90.0 90.0 X Y Z -2.38070107 14.65618706 0.17305121 1.01450169 1
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1.437 1.437 1.437 6 Reconstruction of Actin-coronin in ADP-BeFx state 2 Coronin decorated Actin filament in ADP-BeFx state helical 1:1
The sample was treated with BeFx.
protein Actin Actin, alpha skeletal muscle 0.042 Helix Oryctolagus cuniculus Rabbit false P68135 Muscle protein coronin Coronin-like protein 0.073 helical true Saccharomyces cerevisiae Yeast Q06440 Escherichia coli blot force 1, blot time 4s, blot once, wait time 2s, drain time 2s; sample volume applied to each grid was 2.5 uL ETHANE 100 FEI VITROBOT MARK IV Software FIELD EMISSION GUN 25 BRIGHT FIELD 2000 4000 FLOOD BEAM Nitrogen cooled GATAN ULTRASCAN 4000 (4k x 4k) 2.7 104384 80 FEI TITAN KRIOS 23-OCT-2012 FEI TITAN KRIOS AUTOGRID HOLDER 120 59000 1200 15 16 Chimera rigid body REAL 3MFP 28.23 166.9 C1 LEFT HANDED filament 200 mesh Quantifoil, baked and glow-discharged 7.5
10 mM Tris, 0.2 mM CaCl2, 1mM DTT, 1 mM MgCl2, 0.1 mM BeCl2, mM NaF, 0.2 mM ATP, 50 mM KCl
 helical EMAN EMAN, IHRSR ctffind3, software astigmatism correction 5.1 FSC 0.143, semi-independent
IHRSR