HPUB OBS RCSB RCSB RCSB 2014-02-06 2014-03-19 2014-10-22 2014-10-22 EMD-6100 CryoEM of Actin-Coronin in ADP state Ge P Durer ZAO Kudryashov D Zhou ZH Reisler E Actin, coronin, ADP, cytoskeleton Ge P Durer ZAO Kudryashov D Zhou ZH Reisler E CryoEM reveals different coronin binding modes for ADP- and ADP-BeFx- actin filaments To Be Published Coronin decorated Actin filament in ADP state Coronin decorated Actin filament in ADP state
The sample was aged in the presence of hexokinase to deplete ATP.
helical 1:1 2 Actin Oryctolagus cuniculus Rabbit Muscle 0.042 Helix false P68135 coronin Saccharomyces cerevisiae Yeast 0.073 helical true Escherichia coli Q06440 helical filament 7.5
10 mM Tris, 0.2 mM CaCl2, 1mM DTT, 1 mM MgCl2, 50 mM KCl
200 mesh Quantifoil, baked and glow-discharged
 ETHANE 100 FEI VITROBOT MARK IV blot force 1, blot time 4s, blot once, wait time 2s, drain time 2s; sample volume applied to each grid was 2.5 uL FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 120 2.7 2.0 4.0 59000.0 104384.0 FEI TITAN KRIOS AUTOGRID HOLDER 80 Software 2012-12-12 GATAN ULTRASCAN 4000 (4k x 4k) 15 1380 25 16.0 Nitrogen cooled
IHRSR
28.18 165.9 C1 OTHER 6.1 OTHER EMAN, IHRSR
ctffind3, software astigmatism correction
 emd_5893.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 -160 -160 -160 320 320 320 459.84003 459.84003 459.84003 90.0 90.0 90.0 X Y Z -2.28358412 14.86220837 0.18626508 1.09664905 1.437 1.437 1.437 7.0 AUTHOR Reconstruction of Actin-coronin in ADP state
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 3MFP RIGID BODY FIT Chimera REAL