<?xml version="1.0" encoding="UTF-8"?>
<emd emdb_id="EMD-5827" version="3.0.1.9">
    <admin>
        <status_history_list>
            <status status_id="1">
                <code>REL</code>
            </status>
        </status_history_list>
        <current_status>
            <code>OBS</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2013-12-02</deposition>
            <header_release>2013-12-25</header_release>
            <map_release>2014-12-10</map_release>
            <obsolete>2014-12-10</obsolete>
            <update>2014-12-10</update>
        </key_dates>
        <title>APO conformation of the bacteriophage EL encoded chaperonin</title>
        <authors_list>
            <author>Molugu SK</author>
            <author>Hildenbrand ZL</author>
            <author>Tafoya DA</author>
            <author>Herrera N</author>
            <author>Enriquez AS</author>
            <author>Morgan DG</author>
            <author>Sherman MB</author>
            <author>He L</author>
            <author>Georgopoulos C</author>
            <author>Sernova NV</author>
            <author>Kurochkina LP</author>
            <author>Mesyanzhinov VV</author>
            <author>Miroshnikov KA</author>
            <author>Bernal RA</author>
        </authors_list>
        <keywords>chaperonin, bacteriophage EL</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Molugu SK</author>
                    <author order="2">Hildenbrand ZL</author>
                    <author order="3">Tafoya DA</author>
                    <author order="4">Herrera N</author>
                    <author order="5">Enriquez AS</author>
                    <author order="6">Morgan DG</author>
                    <author order="7">Sherman MB</author>
                    <author order="8">He L</author>
                    <author order="9">Georgopoulos C</author>
                    <author order="10">Sernova NV</author>
                    <author order="11">Kurochkina LP</author>
                    <author order="12">Mesyanzhinov VV</author>
                    <author order="13">Miroshnikov KA</author>
                    <author order="14">Bernal RA</author>
                    <title>Ring separation highlights the protein folding mechanism used by the phage EL encoded chaperonin</title>
                    <journal>To Be Published</journal>
                </journal_citation>
            </primary_citation>
        </citation_list>
    </crossreferences>
    <sample>
        <name>Bacteriophage EL encoded chaperonin in the apo conformation</name>
        <supramolecule_list>
            <sample_supramolecule supramolecule_id="1000">
                <name>Bacteriophage EL encoded chaperonin in the apo conformation</name>
                <oligomeric_state>tetradecamer</oligomeric_state>
                <number_unique_components>1</number_unique_components>
            </sample_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>chaperonin</name>
                <natural_source database="NCBI">
                    <organism ncbi="273133">Pseudomonas phage EL</organism>
                </natural_source>
                <oligomeric_state>dodecamer</oligomeric_state>
                <recombinant_exp_flag>true</recombinant_exp_flag>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="562">Escherichia coli</recombinant_organism>
                    <recombinant_strain>BL21</recombinant_strain>
                </recombinant_expression>
                <sequence/>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <concentration units="mg/mL">2.5</concentration>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>FEI VITROBOT MARK III</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>JEOL 2200FS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <nominal_magnification>60000.0</nominal_magnification>
                    <specimen_holder_model>GATAN LIQUID NITROGEN</specimen_holder_model>
                    <date>2006-08-05</date>
                    <image_recording_list>
                        <image_recording>
                            <film_or_detector_model category="CCD">GATAN ULTRASCAN 4000 (4k x 4k)</film_or_detector_model>
                            <number_real_images>80</number_real_images>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">9.3</resolution>
                    <resolution_method>OTHER</resolution_method>
                    <software_list>
                        <software>
                            <name>MRC, EMAN</name>
                        </software>
                    </software_list>
                    <number_images_used>65000</number_images_used>
                </final_reconstruction>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="16001">
        <file>emd_5827.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>160</col>
            <row>160</row>
            <sec>160</sec>
        </dimensions>
        <origin>
            <col>-80</col>
            <row>-80</row>
            <sec>-80</sec>
        </origin>
        <spacing>
            <x>160</x>
            <y>160</y>
            <z>160</z>
        </spacing>
        <cell>
            <a units="Å">315.2</a>
            <b units="Å">315.2</b>
            <c units="Å">315.2</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-9.82347679</minimum>
            <maximum>7.84446955</maximum>
            <average>0.00177792</average>
            <std>0.60859835</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">1.97</x>
            <y units="Å">1.97</y>
            <z units="Å">1.97</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>1.4</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <annotation_details>Reconstruction of phage EL chaperonin in apo conformation</annotation_details>
        <details>::::EMDATABANK.org::::EMD-5827::::</details>
    </map>
    <interpretation>
        <figure_list>
            <figure>
                <file>emd_5827.jpg</file>
            </figure>
        </figure_list>
    </interpretation>
</emd>
