HPUB OBS PDBj RCSB PDBj 2013-04-07 2013-10-16 2014-06-25 2014-06-25 Tip49b DomainII truncated dodecameric human Tip49a-Trx/Tip49b complex Song F Zhu P Tip49a, Tip49b, Ruvbl1, Ruvbl2, Pontin, Reptin, AMP-PNP, AAA+, Domain II, ATPase, Chromatin remodelling, Thioredoxin Song F Zhu P Cryo-EM structure of full-length dodecameric human Tip49a/Tip49b complex To Be Published Chimeric Tip49b DomainII truncated dodecameric human Tip49a-Trx/Tip49b complex Chimeric Tip49b DomainII truncated dodecameric human Tip49a-Trx/Tip49b complex One homohexamer of Tip49a-Trx binds to one homohexamer of DomainII truncated Tip49b 3 0.66 Tip49a Homo sapiens Human Nucleus 0.05 6 Hexamer true Escherichia coli Rosetta (DE3) pLysS pRSF Duet-1 Q9Y265 GO:0035267 IPR003593 Tip49b Escherichia coli E.coli 0.04
Residues Glu133-Val238 within DomainII were replaced with an Ala-Gly-Ala linker
6 Hexamer true Escherichia coli Rosetta (DE3) pLysS pRSF Duet-1 Q9Y230 GO:0032508 IPR010339 Thioredoxin Escherichia coli E.coli 0.012
Fused to the C-terminus of Tip49a
6 Hexamer true Escherichia coli Rosetta (DE3) pLysS pRSF Duet-1 singleParticle particle 3.1 8.0
200mM NaCl, 25mM Tris-HCl, 1mM PMSF, 1mM DTT
 NEGATIVE
3.5ul sample on grid stained with 2% w/v uranyl acetate for 40 seconds
 NONE OTHER FEI TECNAI 20 FLOOD BEAM BRIGHT FIELD LAB6 200 2.5 50000.0 SIDE ENTRY, EUCENTRIC 2013-03-05 GATAN ULTRASCAN 1000 (2k x 2k) 101 OTHER EMAN1, EMAN2 2789 emd_5636.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 140 140 140 -70 -70 -70 140 140 140 280.0 280.0 280.0 90.0 90.0 90.0 X Y Z -5.02168798 10.19056797 0.0 0.99999982 2.0 2.0 2.0 2.85 AUTHOR Reconstruction of Chimeric Tip49b DomainII truncated Tip49a-Trx/Tip49b dodecameric complex
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