2014-06-25 OBS 2013-04-06 RCSB PDBj 2013-10-16 2014-06-25
The hold limit has been exceeded.
Tip49a DomainII truncated dodecameric human Tip49a-Trx/Tip49b complex Song F, Zhu P Tip49a, Tip49b, Ruvbl1, Ruvbl2, Pontin, Reptin, AMP-PNP, AAA+, Domain II, ATPase, Chromatin remodelling, Thioredoxin Song F, Zhu P Cryo-EM structure of full-length dodecameric human Tip49a/Tip49b complex To Be Published emd_5635.map.gz Image stored as Reals 140 140 140 -70 -70 -70 69 69 69 140 140 140 280.0 280.0 280.0 90.0 90.0 90.0 X Y Z -5.82603455 11.12076569 0E-8 0.99999982 1
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2.0 2.0 2.0 2.5 Reconstruction of Chimeric Tip49a DomainII truncated Tip49a-Trx/Tip49b dodecameric complex
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3 Chimeric Tip49a DomainII truncated dodecameric human Tip49a-Trx/Tip49b complex One homohexamer of Tip49a DomainII truncated Tip49a-Trx binds to one homohexamer of Tip49b 0.66 protein Tip49a Ruvbl1, Pontin, TIP49, ECP54, INO80H 0.04
Residues Glu126-Ile234 within DomainII were replaced with an Ala-Gly-Ala linker
Homo sapiens Human 6 true Hexamer Q9Y265 GO:0035267 IPR003593 Nucleus pRSF Duet-1 Rosetta (DE3) pLysS Escherichia coli protein Tip49b Ruvbl2, Reptin, TIP48, ECP51, INO80J 0.05 true Escherichia coli Hexamer 6 E.coli IPR010339 GO:0032508 Q9Y230 Rosetta (DE3) pLysS Escherichia coli pRSF Duet-1 protein Thioredoxin Trx 0.012
Fused to the C-terminus of Tip49a
Escherichia coli 6 E.coli Hexamer true pRSF Duet-1 Escherichia coli Rosetta (DE3) pLysS NONE NONE LAB6 BRIGHT FIELD FLOOD BEAM GATAN ULTRASCAN 1000 (2k x 2k) 2.5 FEI TECNAI 20 05-MAR-2013 SIDE ENTRY, EUCENTRIC 200 50000 96 3.5ul sample on grid stained with 2% w/v uranyl acetate for 40 seconds particle 3.5
200mM NaCl, 25mM Tris-HCl, 1mM PMSF, 1mM DTT
8.0 singleParticle Projection matching and Fourier reconstruction EMAN1, EMAN2 C1 2811