2014-06-25 OBS 2013-04-06 RCSB PDBj 2013-10-16 2014-06-25
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Cryo-EM structure of full-length dodecameric human Tip49a-Trx/Tip49b complex Song F, Zhu P Tip49a, Tip49b, Ruvbl1, Ruvbl2, Pontin, Reptin, AMP-PNP, AAA+, Domain II, ATPase, Chromatin remodelling, Thioredoxin Song F, Zhu P Cryo-EM structure of full-length dodecameric human Tip49a/Tip49b complex To Be Published emd_5634.map.gz Image stored as Reals 260 260 260 -32 -32 -32 227 227 227 260 260 260 520.0 520.0 520.0 90.0 90.0 90.0 X Y Z -23.59035873 32.87937927 0E-8 0.99999994 1
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2.0 2.0 2.0 9.15 Reconstruction of Chimeric Tip49a-Trx/Tip49b dodecameric complex
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3 Chimeric human chromatin remodelling Tip49a-Trx/Tip49b complex One homohexamer of Tip49a-Trx binds to one homohexamer of Tip49b 0.74 protein Tip49a Ruvbl1, Pontin, TIP49, ECP54, INO80H 0.05 Homo sapiens Human 6 true Hexamer Q9Y265 GO:0035267 IPR003593 Nucleus pRSF Duet-1 Rosetta (DE3) pLysS Escherichia coli protein Thioredoxin Trx 0.012
Fused to the C-terminal of Tip49a
true Escherichia coli Hexamer 6 E.coli Rosetta (DE3) pLysS Escherichia coli pRSF Duet-1 protein Tip49b Ruvbl2, Reptin, TIP48, ECP51, INO80J 0.05 Homo sapiens 6 Human Hexamer true Q9Y230 IPR010339 GO:0032508 Nucleus pRSF Duet-1 Escherichia coli Rosetta (DE3) pLysS Blot for 4.5 seconds before plunging ETHANE 100 FEI VITROBOT MARK IV 100 LAB6 20 BRIGHT FIELD 1500 85 3900 FLOOD BEAM Gatan CT3500 GATAN ULTRASCAN 1000 (2k x 2k) 2.5 FEI TECNAI 20 01-NOV-2011 GATAN LIQUID NITROGEN 200 50000 230 Chimera rigid body REAL 3J3J A B particle 1 Quantifoil R2/1 300 mesh grid
200mM NaCl, 25mM Tris-HCl, 1mM PMSF, 1mM DTT, 4mM MgCl2, 3mM AMP-PNP
8.0 singleParticle Projection matching and Fourier reconstruction EMAN1, EMAN2 CTF correction of each whole micrograph 19 FSC 0.5 1643 C1 8825