HPUB OBS PDBj RCSB PDBj 2013-04-06 2013-10-16 2014-06-25 2014-06-25 Cryo-EM structure of full-length dodecameric human Tip49a-Trx/Tip49b complex Song F Zhu P Tip49a, Tip49b, Ruvbl1, Ruvbl2, Pontin, Reptin, AMP-PNP, AAA+, Domain II, ATPase, Chromatin remodelling, Thioredoxin Song F Zhu P Cryo-EM structure of full-length dodecameric human Tip49a/Tip49b complex To Be Published Chimeric human chromatin remodelling Tip49a-Trx/Tip49b complex Chimeric human chromatin remodelling Tip49a-Trx/Tip49b complex One homohexamer of Tip49a-Trx binds to one homohexamer of Tip49b 3 0.74 Tip49a Homo sapiens Human Nucleus 0.05 6 Hexamer true Escherichia coli Rosetta (DE3) pLysS pRSF Duet-1 Q9Y265 GO:0035267 IPR003593 Thioredoxin Escherichia coli E.coli 0.012
Fused to the C-terminal of Tip49a
6 Hexamer true Escherichia coli Rosetta (DE3) pLysS pRSF Duet-1 Tip49b Homo sapiens Human Nucleus 0.05 6 Hexamer true Escherichia coli Rosetta (DE3) pLysS pRSF Duet-1 Q9Y230 GO:0032508 IPR010339 singleParticle particle 1 8.0
200mM NaCl, 25mM Tris-HCl, 1mM PMSF, 1mM DTT, 4mM MgCl2, 3mM AMP-PNP
Quantifoil R2/1 300 mesh grid
 ETHANE 100 100 FEI VITROBOT MARK IV Blot for 4.5 seconds before plunging FEI TECNAI 20 FLOOD BEAM BRIGHT FIELD LAB6 200 2.5 1.5 3.9 50000.0 GATAN LIQUID NITROGEN 85 2011-11-01 GATAN ULTRASCAN 1000 (2k x 2k) 230 20 Gatan CT3500
CTF correction of each whole micrograph
 OTHER 19.0 FSC 0.5 CUT-OFF EMAN1, EMAN2 8825 1643 emd_5634.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 260 260 260 -32 -32 -32 260 260 260 520.0 520.0 520.0 90.0 90.0 90.0 X Y Z -23.59035873 32.87937927 0.0 0.99999994 2.0 2.0 2.0 9.15 AUTHOR Reconstruction of Chimeric Tip49a-Trx/Tip49b dodecameric complex
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 3J3J B RIGID BODY FIT Chimera REAL
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