2014-06-25 OBS 2013-03-08 RCSB PDBj 2013-05-08 2014-06-25
Author request Related pdb entry 3j3j is withdrawn.
Cryo-EM structure of full-length dodecameric human Tip49a/Tip49b complex in AMP-PNP-bound state Song F, Zhu P Tip49a, Tip49b, Ruvbl1, Ruvbl2, Pontin, Reptin, AMP-PNP, AAA+, Domain II, ATPase, Chromatin remodelling 3j3j Song F, Zhu P Cryo-EM structure of full-length dodecameric human Tip49a/Tip49b complex To Be Published emd_5599.map.gz Image stored as Reals 240 240 240 -120 -120 -120 119 119 119 240 240 240 287.04 287.04 287.04 90.0 90.0 90.0 X Y Z -13.09076977 22.14615059 0.03302019 0.99216431 1
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1.196 1.196 1.196 5.4 Reconstruction of full-length dodecameric human Tip49a/Tip49b complex bound with AMP-PNP
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emd_5599_fsc.xml 2 Human chromatin remodelling Tip49a/Tip49b complex One homohexamer of Tip49a binds to one homohexamer of Tip49b 0.66 protein Tip49a Ruvbl1, Pontin, TIP49, ECP54, INO80H 0.05 6 hexamer Homo sapiens Human true GO:0035267 Q9Y265 IPR003593 Nucleus Escherichia coli BL21 Rosetta (DE3) pLysS pRSF Duet-1 protein Tip49b Ruvbl2, Reptin, TIP48, ECP51, INO80J 0.05 hexamer true 6 Homo sapiens Human IPR010339 Q9Y230 GO:0032508 Nucleus pRSF Duet-1 Escherichia coli BL21 Rosetta (DE3) pLysS Blot for 4 seconds before plunging ETHANE 100 FEI VITROBOT MARK IV 100 Objective lens astigmatism was corrected at 155,000 times magnification FIELD EMISSION GUN 20 BRIGHT FIELD 1600 85 3500 FLOOD BEAM Liquid nitrogen cooled
Parallel beam illumination
GATAN ULTRASCAN 4000 (4k x 4k) 2.7 0 0 FEI TITAN KRIOS 05-JUL-2011 FEI TITAN KRIOS AUTOGRID HOLDER 300 75000 1231 15 particle 3 C-flat 22-4C 400 mesh copper grid
200mM NaCl, 25mM Tris-HCl, 1mM PMSF, 1mM DTT, 4mM MgCl2, 3mM AMP-PNP
8.0 singleParticle Projection matching and Fourier reconstruction EMAN1, EMAN2, XMIPP CTF correction of each whole micrograph
The final map was deconvolved by a B-factor of 134 angstrom^2 weighed by the FSC curve. Low-pass filtration to 3.2 angstrom was applied to the final reconstruction.
3.9 FSC 0.143, gold-standard 4755 C6 71782