HPUB OBS PDBj RCSB PDBj 2013-03-08 2013-05-08 2014-06-25 2014-06-25 Cryo-EM structure of full-length dodecameric human Tip49a/Tip49b complex in AMP-PNP-bound state Song F Zhu P Tip49a, Tip49b, Ruvbl1, Ruvbl2, Pontin, Reptin, AMP-PNP, AAA+, Domain II, ATPase, Chromatin remodelling Song F Zhu P Cryo-EM structure of full-length dodecameric human Tip49a/Tip49b complex To Be Published 3j3j FULLOVERLAP Human chromatin remodelling Tip49a/Tip49b complex Human chromatin remodelling Tip49a/Tip49b complex One homohexamer of Tip49a binds to one homohexamer of Tip49b 2 0.66 Tip49a Homo sapiens Human Nucleus 0.05 6 hexamer true Escherichia coli BL21 Rosetta (DE3) pLysS pRSF Duet-1 Q9Y265 GO:0035267 IPR003593 Tip49b Homo sapiens Human Nucleus 0.05 6 hexamer true Escherichia coli BL21 Rosetta (DE3) pLysS pRSF Duet-1 Q9Y230 GO:0032508 IPR010339 singleParticle particle 3 8.0
200mM NaCl, 25mM Tris-HCl, 1mM PMSF, 1mM DTT, 4mM MgCl2, 3mM AMP-PNP
C-flat 22-4C 400 mesh copper grid
 ETHANE 100 100 FEI VITROBOT MARK IV Blot for 4 seconds before plunging FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.6 3.5 75000.0 FEI TITAN KRIOS AUTOGRID HOLDER 85 Objective lens astigmatism was corrected at 155,000 times magnification
Parallel beam illumination
2011-07-05 GATAN ULTRASCAN 4000 (4k x 4k) 15 1231 20 Liquid nitrogen cooled 0 0
CTF correction of each whole micrograph
 OTHER 3.9 OTHER EMAN1, EMAN2, XMIPP
The final map was deconvolved by a B-factor of 134 angstrom^2 weighed by the FSC curve. Low-pass filtration to 3.2 angstrom was applied to the final reconstruction.
71782 4755 emd_5599.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 240 240 240 -120 -120 -120 240 240 240 287.04 287.04 287.04 90.0 90.0 90.0 X Y Z -13.09076977 22.14615059 0.03302019 0.99216431 1.196 1.196 1.196 5.4 AUTHOR Reconstruction of full-length dodecameric human Tip49a/Tip49b complex bound with AMP-PNP
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