2014-03-19 OBS 2013-01-11 RCSB RCSB 2013-07-03 2014-03-19
Author requested withdrawal of entry.
EMD-5575 Electron cryo-microscopy of murine p97WT Mountassif D, Fabre L, Zaid Y, Halawani D, Bostina M, Rouiller I p97, mutant, hIBMPFTD and Cryo-EM. Mountassif D, Fabre L, Zaid Y, Halawani D, Bostina M, Rouiller I Impact of the mutation R155P implicated in hIBMPFTD diseases on the conformational change of D2-domain of the AAA ATPase p97/VCP To Be Published emd_5574.map.gz Image stored as Reals 128 128 128 -64 -64 -64 63 63 63 128 128 128 294.4 294.4 294.4 90.0 90.0 90.0 X Y Z -0.02718199 0.03536404 0.00040384 0.00325693 1
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2.3 2.3 2.3 0.017 Reconstruction of p97WT 1 p97 WT homohexamer 0.6
The sample was very pure and homogenous.
Gel filtration and Polyacrylamide gel electrophoresis 0.6 protein Transitional endoplasmic reticulum ATPase vcp 0.6 0.6 Mus musculus true hexamer mouse 6 Q01853 endoplasmic reticulum cytoplasm pTrcHis2C Escherichia coli BL21(DE3) Blot 2 seconds, blot force 1 ETHANE
Vitrification carried out in ethane in liquid nitrogen atmosphere.
100 FEI VITROBOT MARK IV 120 Objective lens astigmatism was corrected at 50,000 times magnification FIELD EMISSION GUN 15 FEI BRIGHT FIELD 1000 78 3500 FLOOD BEAM 108 liquid nitrogen GENERIC CCD 103 FEI TECNAI F20 13-SEP-2011 GATAN LIQUID NITROGEN 200 50000 80
The domains were symmetrically fitted into the map using the Molecular Dynamics Flexible Fitting software.
Chimera flexible REAL 3CF1 A B C particle 2.5 400 mesh copper grid
20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 2.5 mM DTT
7.5 singleParticle Xmipp ctffind3 14 FSC 0.5 64 C6 19700
Particles in multiple orientations were selected from cryo-micrographs using Signature and analyzed by XMipp software.