<?xml version="1.0" encoding="UTF-8"?>
<emdEntry accessCode="5518" version="1.9.6">
    <admin>
        <lastUpdate>2013-12-25</lastUpdate>
    </admin>
    <deposition>
        <status prior="REL">OBS</status>
        <depositionDate>2012-10-05</depositionDate>
        <depositionSite>RCSB</depositionSite>
        <processingSite>RCSB</processingSite>
        <headerReleaseDate>2012-11-28</headerReleaseDate>
        <mapReleaseDate>2012-11-28</mapReleaseDate>
        <obsoletedDate>2013-12-25</obsoletedDate>
        <supersededByList>
            <entry>EMD-5801</entry>
        </supersededByList>
        <details>New map has higher resolution.</details>
        <title>eRF1-eRF3-bound 80S ribosome</title>
        <authors>Taylor DJ, Unbehaun A, Li W, Das S, Lei J, Liao H, Grassucci R, Pestova TV, Frank J</authors>
        <keywords>80S ribosome, electron density map, image processing, polypeptide release factors</keywords>
        <fittedPDBEntryIdList>
            <fittedPDBEntryId>3j2k</fittedPDBEntryId>
        </fittedPDBEntryIdList>
        <primaryReference published="true">
            <journalArticle>
                <authors>Taylor D, Unbehaun A, Li W, Das S, Lei J, Liao HY, Grassucci RA, Pestova TV, Frank J</authors>
                <articleTitle>Cryo-EM structure of the mammalian eukaryotic release factor eRF1-eRF3-associated termination complex.</articleTitle>
                <journal>PROC.NAT.ACAD.SCI.USA</journal>
                <volume>109</volume>
                <firstPage>18413</firstPage>
                <lastPage>18418</lastPage>
                <year>2012</year>
                <externalReference type="pubmed">23091004</externalReference>
                <externalReference type="doi">doi:10.1073/pnas.1216730109</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="13719" type="map">emd_5518.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numColumns>152</numColumns>
            <numRows>152</numRows>
            <numSections>152</numSections>
        </dimensions>
        <origin>
            <originCol>-76</originCol>
            <originRow>-76</originRow>
            <originSec>-76</originSec>
        </origin>
        <limit>
            <limitCol>75</limitCol>
            <limitRow>75</limitRow>
            <limitSec>75</limitSec>
        </limit>
        <spacing>
            <spacingCol>152</spacingCol>
            <spacingRow>152</spacingRow>
            <spacingSec>152</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">456.0</cellA>
            <cellB units="A">456.0</cellB>
            <cellC units="A">456.0</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-1.52693391</minimum>
            <maximum>3.59920382</maximum>
            <average>0.05825989</average>
            <std>0.38245708</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details>::::EMDATABANK.org::::EMD-5518::::</details>
        <pixelSpacing>
            <pixelX units="A">3</pixelX>
            <pixelY units="A">3</pixelY>
            <pixelZ units="A">3</pixelZ>
        </pixelSpacing>
        <contourLevel source="emdb">0.5</contourLevel>
        <annotationDetails>eRF1-eRF3-bound 80S ribosome</annotationDetails>
    </map>
    <supplement>
        <maskSet/>
        <sliceSet/>
        <figureSet/>
        <fscSet/>
    </supplement>
    <sample>
        <numComponents>1</numComponents>
        <name>80S ribosome</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>ribosome-eukaryote</entry>
                <sciName>80S ribosome</sciName>
                <ribosome-eukaryote>
                    <eukaryote>ALL</eukaryote>
                    <synSpeciesName>rabbit</synSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <sciSpeciesName ncbiTaxId="9986">Oryctolagus cuniculus</sciSpeciesName>
                    <externalReferences/>
                    <natSource/>
                    <engSource/>
                </ribosome-eukaryote>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <instrument>FEI VITROBOT MARK I</instrument>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>SPOT SCAN</illuminationMode>
            <microscope>FEI POLARA 300</microscope>
            <date>01-APR-2008</date>
            <specimenHolderModel>GATAN HELIUM</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition>
            <samplingSize units="microns">14</samplingSize>
        </imageAcquisition>
        <fitting/>
        <specimenPreparation>
            <specimenState>particle</specimenState>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <algorithm>single particle projection matching</algorithm>
            <eulerAnglesDetails>15</eulerAnglesDetails>
            <software>Spider</software>
            <ctfCorrection>group defocus</ctfCorrection>
            <resolutionByAuthor>18</resolutionByAuthor>
            <resolutionMethod>FSC</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <numProjections>195432</numProjections>
            <appliedSymmetry>C1</appliedSymmetry>
        </singleParticle>
    </processing>
</emdEntry>
