<?xml version="1.0" encoding="UTF-8"?>
<emd emdb_id="EMD-5518" version="3.0.1.9">
    <admin>
        <status_history_list>
            <status status_id="1">
                <code>REL</code>
            </status>
        </status_history_list>
        <current_status>
            <code superseded="true">OBS</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2012-10-05</deposition>
            <header_release>2012-11-28</header_release>
            <map_release>2012-11-28</map_release>
            <obsolete>2013-12-25</obsolete>
            <update>2013-12-25</update>
        </key_dates>
        <superseded_by_list>
            <entry>
                <entry>EMD-5801</entry>
            </entry>
        </superseded_by_list>
        <title>eRF1-eRF3-bound 80S ribosome</title>
        <authors_list>
            <author>Taylor DJ</author>
            <author>Unbehaun A</author>
            <author>Li W</author>
            <author>Das S</author>
            <author>Lei J</author>
            <author>Liao H</author>
            <author>Grassucci R</author>
            <author>Pestova TV</author>
            <author>Frank J</author>
        </authors_list>
        <keywords>80S ribosome, electron density map, image processing, polypeptide release factors</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Taylor D</author>
                    <author order="2">Unbehaun A</author>
                    <author order="3">Li W</author>
                    <author order="4">Das S</author>
                    <author order="5">Lei J</author>
                    <author order="6">Liao HY</author>
                    <author order="7">Grassucci RA</author>
                    <author order="8">Pestova TV</author>
                    <author order="9">Frank J</author>
                    <title>Cryo-EM structure of the mammalian eukaryotic release factor eRF1-eRF3-associated termination complex.</title>
                    <journal>PROC.NAT.ACAD.SCI.USA</journal>
                    <volume>109</volume>
                    <first_page>18413</first_page>
                    <last_page>18418</last_page>
                    <year>2012</year>
                    <external_references type="PUBMED">23091004</external_references>
                    <external_references type="DOI">doi:10.1073/pnas.1216730109</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>3j2k</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>80S ribosome</name>
        <supramolecule_list>
            <sample_supramolecule supramolecule_id="1000">
                <name>80S ribosome</name>
                <number_unique_components>1</number_unique_components>
            </sample_supramolecule>
            <complex_supramolecule supramolecule_id="1">
                <name>80S ribosome</name>
                <recombinant_exp_flag>false</recombinant_exp_flag>
                <natural_source database="NCBI">
                    <organism ncbi="9986">Oryctolagus cuniculus</organism>
                    <synonym_organism>rabbit</synonym_organism>
                </natural_source>
                <recombinant_expression database="NCBI"/>
                <ribosome-details>ribosome-eukaryote: ALL</ribosome-details>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <instrument>FEI VITROBOT MARK I</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI POLARA 300</microscope>
                    <illumination_mode>SPOT SCAN</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <specimen_holder_model>GATAN HELIUM</specimen_holder_model>
                    <date>2008-04-01</date>
                    <image_recording_list>
                        <image_recording>
                            <digitization_details>
                                <sampling_interval units="µm">14</sampling_interval>
                            </digitization_details>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <ctf_correction>
                    <details>group defocus</details>
                </ctf_correction>
                <final_reconstruction>
                    <algorithm>OTHER</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">18.0</resolution>
                    <resolution_method>OTHER</resolution_method>
                    <software_list>
                        <software>
                            <name>Spider</name>
                        </software>
                    </software_list>
                    <number_images_used>195432</number_images_used>
                </final_reconstruction>
                <final_angle_assignment>
                    <details>15</details>
                </final_angle_assignment>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="13719">
        <file>emd_5518.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>152</col>
            <row>152</row>
            <sec>152</sec>
        </dimensions>
        <origin>
            <col>-76</col>
            <row>-76</row>
            <sec>-76</sec>
        </origin>
        <spacing>
            <x>152</x>
            <y>152</y>
            <z>152</z>
        </spacing>
        <cell>
            <a units="Å">456.0</a>
            <b units="Å">456.0</b>
            <c units="Å">456.0</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-1.52693391</minimum>
            <maximum>3.59920382</maximum>
            <average>0.05825989</average>
            <std>0.38245708</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">3</x>
            <y units="Å">3</y>
            <z units="Å">3</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.5</level>
                <source>EMDB</source>
            </contour>
        </contour_list>
        <annotation_details>eRF1-eRF3-bound 80S ribosome</annotation_details>
        <details>::::EMDATABANK.org::::EMD-5518::::</details>
    </map>
</emd>
