2013-08-14 OBS 2012-02-07 RCSB RCSB 2012-02-16 2013-08-14
This header was removed at author request prior to map release.
C3 symmetric heteromeric oligomer rATcpn-ab in apo state Zhang K, Wang L, Liu YX, Wang X, Gao B, Hu ZJ, Ji G, Chan KY, Schulten K, Dong ZY, Sun F Group II chaperonin, thermosome 3j1l Zhang K, Wang L, Liu YX, Wang X, Gao B, Hu ZJ, Ji G, Chan KY, Schulten K, Dong ZY, Sun F Structural insights into the intra-ring cooperation of the heteromeric group II chaperonin To Be Published emd_5403.map.gz Image stored as Reals 144 144 144 -72 -72 -72 71 71 71 144 144 144 344.448 344.448 344.448 90.0 90.0 90.0 X Y Z -14.90416622 19.07429123 -1E-8 1.69076586 1
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2.392 2.392 2.392 3.8 Reconstruction of the major class of C3 symmetric heteromeric oligomer rATcpn-ab in apo state 1 Heteromeric group II chaperonin rATcpn-ab in apo state octadecamer 1.08 1.08 protein Heteromeric group II chaperonin rATcpn-ab rATcpn-ab 1.08 1.08 Acidianus tengchongensis true octadecamer Archaea 1 S5T PET-23b Escherichia coli Blot for 4 seconds before plunging ETHANE 100 FEI VITROBOT MARK IV 100 Objective lens astigmatism was corrected at 96,000 times magnification FIELD EMISSION GUN 20 BRIGHT FIELD 1500 3500 FLOOD BEAM GATAN ULTRASCAN 4000 (4k x 4k) 2.7 75000 FEI TITAN KRIOS 07-JUN-2010 GATAN LIQUID NITROGEN 300 75000 3000 32
Protocol: Rigid body and Molecular Dynamics Flexible Fitting. symmetry-restrained MDFF
Chimera flexible REAL 3KO1 A 3 400-mesh GiGTM grid with holes of 2 um diameter and 2 um spacing particle
25 mM Tris-HCl, pH 7.5, 12 mM MgCl2, 50 mM KCl
7.5 singleParticle Spider,EMAN,1.9 The whole micrograph 13.3 FSC 0.5 10957