HPUB OBS RCSB RCSB RCSB 2012-02-07 2012-02-16 2013-08-14 2013-08-14 9-fold symmetric rATcpn-alpha in ATP-binding state Zhang K Wang L Liu YX Wang X Gao B Hu ZJ Ji G Chan KY Schulten K Dong ZY Sun F Group II chaperonin, thermosome Zhang K Wang L Liu YX Wang X Gao B Hu ZJ Ji G Chan KY Schulten K Dong ZY Sun F Structural insights into the intra-ring cooperation of the heteromeric group II chaperonin To Be Published rATcpn-alpha in ATP-binding state rATcpn-alpha in ATP-binding state octadecamer 1 1.08 1.08 Group II chaperonin alpha Acidianus tengchongensis S5T Archaea 1.08 1.08 1 octadecamer true Escherichia coli PET-23b singleParticle particle 3 7.5
25 mM Tris-HCl, pH 7.5, 12 mM MgCl2, 50 mM KCl
400-mesh GiGTM grid with holes of 2 um diameter and 2 um spacing
 ETHANE 100 100 FEI VITROBOT MARK IV Blot for 4 seconds before plunging FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.5 3.5 96000.0 96000.0 GATAN LIQUID NITROGEN Objective lens astigmatism was corrected at 96,000 times magnification 2010-05-17 GATAN ULTRASCAN 4000 (4k x 4k) 900 20 32.0
The whole micrograph
 12.2 FSC 0.5 CUT-OFF Spider,EMAN1.9 2933 emd_5394.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 144 144 144 -72 -72 -72 144 144 144 268.704 268.704 268.704 90.0 90.0 90.0 X Y Z -6.15463352 8.41016769 0.09680571 1.06312263 1.866 1.866 1.866 1.6 AUTHOR Reconstruction of the 9-fold symmetric class of rATcpn-alpha in ATP-binding state
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