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    <admin>
        <lastUpdate>2021-11-22</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2019-02-11</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2021-12-01</headerReleaseDate>
        <obsoletedDate>2021-12-01</obsoletedDate>
        <title>CCMV capsids containing asparaginase</title>
        <authors>Klem R, de Ruiter MV, Dombrowe VM, Juan A, Gil-Cantero D, Allende-Ballestero C, Luque D, Caston JR, Cornelissen JJLM</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Klem R, de Ruiter MV, Dombrowe VM, Juan A, Gil-Cantero D, Allende-Ballestero C, Luque D, Caston JR, Cornelissen JJLM</authors>
                <articleTitle>Encapsulation of L-asparaginase in bacterial or virus-based protein cages for the Treatment of Acute Lymphoblastic Leukemia</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="42593" type="map">emd_4607.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>220</numRows>
            <numColumns>220</numColumns>
            <numSections>220</numSections>
        </dimensions>
        <origin>
            <originRow>-110.0</originRow>
            <originCol>-110.0</originCol>
            <originSec>-110.0</originSec>
        </origin>
        <limit>
            <limitRow>109.0</limitRow>
            <limitCol>109.0</limitCol>
            <limitSec>109.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>220</spacingRow>
            <spacingCol>220</spacingCol>
            <spacingSec>220</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">312.4</cellA>
            <cellB units="A">312.4</cellB>
            <cellC units="A">312.4</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.09245808</minimum>
            <maximum>0.19110848</maximum>
            <average>0.0013323824</average>
            <std>0.015243572</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">1.42</pixelX>
            <pixelY units="A">1.42</pixelY>
            <pixelZ units="A">1.42</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.06</contourLevel>
        <annotationDetails>None</annotationDetails>
    </map>
    <sample>
        <numComponents>1</numComponents>
        <name>Cowpea chlorotic mottle virus</name>
        <details>CCMV VLPs loaded with asparaginase</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>virus</entry>
                <sciName>Cowpea chlorotic mottle virus</sciName>
                <molWtExp units="MDa">1.26</molWtExp>
                <details>CCMV VLPs loaded with asparaginase</details>
                <virus>
                    <sciSpeciesName ncbiTaxId="12303">Cowpea chlorotic mottle virus</sciSpeciesName>
                    <empty>false</empty>
                    <enveloped>false</enveloped>
                    <isolate>STRAIN</isolate>
                    <class>VIRUS-LIKE PARTICLE</class>
                    <natSource>
                        <hostSpecies ncbiTaxId="3917">Vigna unguiculata</hostSpecies>
                    </natSource>
                    <engSource>
                        <expSystem ncbiTaxId="3917">Vigna unguiculata</expSystem>
                    </engSource>
                    <shell id="1">
                        <nameElement>CP</nameElement>
                        <diameter units="A">200.0</diameter>
                        <tNumber>1</tNumber>
                    </shell>
                </virus>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <instrument>LEICA EM CPC</instrument>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">30.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <nominalDefocusMin units="nm">1300.0</nominalDefocusMin>
            <nominalDefocusMax units="nm">3300.0</nominalDefocusMax>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON III (4k x 4k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <calibratedMagnification>98591.0</calibratedMagnification>
            <microscope>FEI TALOS ARCTICA</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">200</acceleratingVoltage>
            <nominalMagnification>73000.0</nominalMagnification>
        </imaging>
        <imageAcquisition>
            <numDigitalImages>3786</numDigitalImages>
            <samplingSize units="microns">14.0</samplingSize>
        </imageAcquisition>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">1</specimenConc>
            <buffer>
                <ph>7.5</ph>
                <details>50mM Tris-HCL, 0.3M NaCl, 1mM DTT, pH 7.5</details>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>5.58</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>I</appliedSymmetry>
            <numProjections>237489</numProjections>
        </singleParticle>
    </processing>
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