2019-09-04 OBS 2019-02-01 PDBe PDBe 2019-06-05 2019-09-04 Structure and assembly of the mitochondrial membrane remodelling GTPase Mgm1 Faelber K, Dietrich L, Noel J, Wollweber F, Pfitzner A, Muehleip A, Sanchez R, Kudryashev M, Chiaruttin N, Lilie H, Schleger J, Rosenbaum E, Hessenberger M, Matthaeus C, Noe F, Roux A, van der Laan M, Kuehlbrandt W, Daumke O Faelber K, Dietrich L, Noel J, Wollweber F, Pfitzner A, Muehleip A, Sanchez R, Kudryashev M, Chiaruttin N, Lilie H, Schleger J, Rosenbaum E, Hessenberger M, Matthaeus C, Noe F, Roux A, van der Laan M, Kuehlbrandt W, Daumke O Structure and assembly of the mitochondrial membrane remodelling GTPase Mgm1 to be published 0353 emd_4583.map.gz Image stored as Reals 160 160 160 0.0 0.0 0.0 159.0 159.0 159.0 160 160 160 432.0 432.0 432.0 90.0 90.0 90.0 X Y Z -49.152670000000001 45.277107000000001 -0.037299063 1.9486346 1
2.7 2.7 2.7 0.35 Subtomogram average map filtered to 21 Angstrom resolution and masked 2 Mgm1
short isoform with C- and N-terminal truncations
protein Mgm1
short isoform with C- and N-terminal truncations
Chaetomium thermophilum var. thermophilum DSM 1495 false Escherichia coli protein Mgm1 false ETHANE 70 277.15 FEI VITROBOT MARK IV
FIELD EMISSION GUN 2.0 BRIGHT FIELD 2000.0 4000.0 FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) 2.7 FEI TITAN KRIOS FEI TITAN KRIOS AUTOGRID HOLDER 300 53000.0 particle 5 7.4
20 mM HEPES, 200 mM NaCl, residual MgCl2, 9mM KCl.
 subtomogramAveraging BACK PROJECTION Dynamo CTF determination was done by Gctf and correction was performed by ctfphaseflip from Imod 20.6 FSC 0.143 CUT-OFF subtomogram averaging by cross correlation maximization C1 1792 1