2019-09-04 OBS 2019-02-01 PDBe PDBe 2019-06-05 2019-09-04 Structure and assembly of the mitochondrial membrane remodelling GTPase Mgm1 Faelber K, Dietrich L, Noel J, Wollweber F, Pfitzner A, Muehleip A, Sanchez R, Kudryashev M, Chiaruttin N, Lilie H, Schleger J, Rosenbaum E, Hessenberger M, Matthaeus C, Noe F, Roux A, van der Laan M, Kuehlbrandt W, Daumke O Faelber K, Dietrich L, Noel J, Wollweber F, Pfitzner A, Muehleip A, Sanchez R, Kudryashev M, Chiaruttin N, Lilie H, Schleger J, Rosenbaum E, Hessenberger M, Matthaeus C, Noe F, Roux A, van der Laan M, Kuehlbrandt W, Daumke O Structure and assembly of the mitochondrial membrane remodelling GTPase Mgm1 to be published 0353 emd_4582.map.gz Image stored as Reals 120 120 120 0.0 0.0 0.0 119.0 119.0 119.0 120 120 120 324.0 324.0 324.0 90.0 90.0 90.0 X Y Z -3.9019773 8.722034000000001 0.33431122 1.2417784 1
2.7 2.7 2.7 2.0 Subtomogram average map filtered to 15 Angstroms 2 Mgm1
short isoform with C- and N-terminal truncations
protein Mgm1
short isoform with C- and N-terminal truncations
Chaetomium thermophilum false Escherichia coli protein Mgm1 Chaetomium thermophilum false Escherichia coli ETHANE 70 277.15 FEI VITROBOT MARK IV
FIELD EMISSION GUN 2.0 BRIGHT FIELD 2000.0 4000.0 FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) 2.7 FEI TITAN KRIOS FEI TITAN KRIOS AUTOGRID HOLDER 300 53000.0 particle 5 7.4
20 mM HEPES, 200 mM NaCl, residual MgCl2, 9mM KCl.
 subtomogramAveraging BACK PROJECTION Dynamo CTF determination was done by Gctf and correction was performed by ctfphaseflip from IMOD 14.7 FSC 0.143 CUT-OFF
Half sets were generated not even-odd but as upper- and lower-halves of particles in given tomograms
Subtomogram averaging by cross correlation maximization C1 11474 1